Olsen Timothy W, Liao Albert, Robinson Hershonna S, Palejwala Neal V, Sprehe Nicholas
Mayo Clinic, Department of Ophthalmology, Rochester, Minnesota, United States.
Emory Eye Center, Emory University Department of Ophthalmology, Atlanta, Georgia, United States.
Invest Ophthalmol Vis Sci. 2017 Oct 1;58(12):5497-5506. doi: 10.1167/iovs.17-22161.
To refine the Minnesota Grading System (MGS) using definitions from the Age-Related Eye Disease Studies (AREDS) into a nine-step grading scale (MGS-9).
A nine-step grading scale descriptive analysis using three key phenotypic features (total drusen area, increased, and decreased pigmentation) of human eyebank eyes that were graded according to definitions from the AREDS criteria in order to harmonize studies of disease progression for research involving human tissue. From 2005 through February 2017, we have analyzed 1159 human eyes, procured from two eyebanks. Each macula was imaged using high-resolution, stereoscopic color fundus photography with both direct- and transillumination. Fundus images were digitally overlaid with a grading template and triangulated for foveal centration.
We documented and stratified risk for each globe by applying the AREDS nine-step grading scale to the key clinical features from the MGS-9. We found a good distribution within the MGS categories (1-9) with few level eight globes. Eyes were processed within 12.1 ± 6.3, hours from the time of death through imaging, dissection, and freezing or fixation. Applying the MGS-9 to 331 pairs (662 eyes were simultaneously graded), 84% were within one-grading step and 93% within two steps of the fellow eye. We also document reticular pseudodrusen, basal laminar drusen, and pattern dystrophy.
The MGS nine-step grading scale enables researchers using human tissue to refine the risk assessment of donor tissue. This analysis will harmonize results among researchers when grading human tissue using MGS criteria. Most importantly, the MGS-9 links directly to the known risk for progression from the AREDS.
利用年龄相关性眼病研究（AREDS）的定义，将明尼苏达分级系统（MGS）细化为一个九步分级量表（MGS - 9）。
采用九步分级量表描述性分析方法，对人类眼库眼中的三个关键表型特征（玻璃膜疣总面积、色素沉着增加和减少）进行分析，这些眼睛是根据AREDS标准的定义进行分级的，以便统一涉及人体组织研究的疾病进展研究。从2005年到2017年2月，我们分析了从两个眼库获取的1159只人眼。每个黄斑区都使用高分辨率立体彩色眼底摄影进行成像，包括直接照明和透照。眼底图像通过数字方式与分级模板叠加，并进行三角测量以确定黄斑中心凹位置。
我们通过将AREDS九步分级量表应用于MGS - 9的关键临床特征，记录并分层了每个眼球的风险。我们发现MGS类别（1 - 9）内分布良好，八级眼球较少。从死亡时间到成像、解剖以及冷冻或固定，眼睛在12.1±6.3小时内完成处理。将MGS - 9应用于331对眼睛（同时对662只眼睛进行分级），84%的眼睛与对侧眼相差一级，93%相差两级。我们还记录了网状假性玻璃膜疣、基底膜玻璃膜疣和图案性营养不良。
MGS九步分级量表使使用人体组织的研究人员能够细化对供体组织的风险评估。当使用MGS标准对人体组织进行分级时，该分析将使研究人员之间的结果更加统一。最重要的是，MGS - 9直接与AREDS中已知的进展风险相关联。
