小鼠模型中红细胞参数及血红蛋白病的分析方法

Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models.

作者信息

Trudel Marie, Sedzro Josepha-Clara

机构信息

Molecular Genetics and Development, Institut de Recherches Cliniques de Montréal, Faculté de Médecine, Université de Montréal, 110 avenue des Pins ouest, Montreal, QC, Canada, H2W 1R7.

出版信息

Methods Mol Biol. 2018;1698:103-115. doi: 10.1007/978-1-4939-7428-3_6.

DOI:10.1007/978-1-4939-7428-3_6

PMID:29076086

Abstract

Over the last decades several mouse models of human hemoglobin disorders have been and continue to be generated. This chapter aims at describing various approaches to evaluate the global red blood cell properties in mouse models of human hemoglobin disorders, in particular thalassemia and sickle cell disease. Analysis of erythroid parameters provides insights into the RBC physiologic or pathophysiologic status. Mice expressing both murine and human globin genes can be investigated using adapted protocols provided herein.

摘要

在过去几十年中，已经建立了多种人类血红蛋白疾病的小鼠模型，并且仍在不断产生新的模型。本章旨在描述评估人类血红蛋白疾病小鼠模型（特别是地中海贫血和镰状细胞病）中全球红细胞特性的各种方法。对红系参数的分析有助于深入了解红细胞的生理或病理生理状态。可以使用本文提供的适配方案对同时表达小鼠和人类珠蛋白基因的小鼠进行研究。