人脐带血来源的红系祖细胞（HUDEP）细胞系的培养与基因操作
Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines.
作者信息
Vinjamur Divya S, Bauer Daniel E
机构信息
Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA.
Dana-Farber Cancer Institute, Harvard Medical School, Harvard Stem Cell Institute, Karp 8211, 1 Blackfan Circle, Boston, MA, 02115, USA.
出版信息
Methods Mol Biol. 2018;1698:275-284. doi: 10.1007/978-1-4939-7428-3_17.
The recently established human umbilical cord blood-derived erythroid progenitor (HUDEP) cell lines have equipped red blood cell researchers with valuable in vitro models of erythroid development. Of the three established HUDEP cell lines, HUDEP-2 cells express predominantly adult β-globin and most closely resemble adult erythroid cells. This chapter describes culture protocols for the maintenance and erythroid differentiation of HUDEP-2 cells. Methods to genetically manipulate HUDEP-2 cells using a CRISPR/Cas9 nuclease-based approach are also discussed.
最近建立的人脐带血来源的红系祖细胞（HUDEP）细胞系，为红细胞研究人员提供了宝贵的红系发育体外模型。在已建立的三个HUDEP细胞系中，HUDEP-2细胞主要表达成人β-珠蛋白，与成人红系细胞最为相似。本章描述了HUDEP-2细胞的培养和红系分化的培养方案。还讨论了使用基于CRISPR/Cas9核酸酶的方法对HUDEP-2细胞进行基因操作的方法。
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