Yu Ho Song, Park Jinju, Lee Hyun Suk, Park Su A, Lee Dong Weon, Park Kwangsung
Department of Urology, Chonnam National University Medical School, Chonnam National University Sexual Medicine Research Center, Gwangju, Korea.
Nano Convergence and Manufacturing Systems Research Division, Korea Institute of Machinery and Materials (KIMM), Daejeon, Korea.
World J Mens Health. 2018 Jan;36(1):66-72. doi: 10.5534/wjmh.17025. Epub 2017 Oct 25.
To investigate the feasibility of a polycaprolactone (PCL) scaffold fabricated by three-dimensional (3D) printing for tissue engineering applications for tunica albuginea.
PCL scaffolds were fabricated by use of a 3D printing system. Two scaffolds were fabricated that differed in the architecture of the lay-down pattern: a 90°PCL scaffold and a 45°PCL scaffold. Mechanical properties were measured to compare tensile strength between the two scaffold types. The scaffolds were characterized by scanning electron microscope (SEM) images. The scaffolds were seeded with fibroblast cells, and the ability of these scaffolds to support the cells was evaluated by immunofluorescence staining.
The PCL scaffolds had well-structured shapes, regular arrays, and good interconnection in SEM images. The horizontal and vertical Young's modulus coefficients were 13 and 12 MPa for the 90°PCL scaffold and 19 and 21 MPa for the 45°PCL scaffold, respectively. Microscopy images revealed that human fibroblast cells covered the entire scaffold surface. Immunofluorescence staining of ER-TR7 confirmed that the fibroblast cells remained viable and proliferated throughout the time course of the culture.
This preliminary study provides experimental evidence for the feasibility of 3D printing of PCL scaffolds for tissue engineering applications of tunica albuginea.
研究通过三维（3D）打印制造的聚己内酯（PCL）支架用于白膜组织工程应用的可行性。
使用3D打印系统制造PCL支架。制造了两种在铺层图案结构上不同的支架：90°PCL支架和45°PCL支架。测量力学性能以比较两种支架类型之间的拉伸强度。通过扫描电子显微镜（SEM）图像对支架进行表征。将成纤维细胞接种到支架上，并通过免疫荧光染色评估这些支架支持细胞的能力。
在SEM图像中，PCL支架具有结构良好的形状、规则的阵列和良好的互连性。90°PCL支架的水平和垂直杨氏模量系数分别为13和12 MPa，45°PCL支架的分别为19和21 MPa。显微镜图像显示人成纤维细胞覆盖了整个支架表面。ER-TR7的免疫荧光染色证实，在整个培养过程中，成纤维细胞保持存活并增殖。
这项初步研究为3D打印PCL支架用于白膜组织工程应用的可行性提供了实验证据。
