Rossi Heini S, Koho Ninna M, Ilves Mika, Rajamäki Minna M, Mykkänen Anna K
Am J Vet Res. 2017 Nov;78(11):1329-1337. doi: 10.2460/ajvr.78.11.1329.
OBJECTIVE To examine whether expression of extracellular matrix metalloproteinase inducer (EMMPRIN) can be detected in equine lungs and whether it correlates with matrix metalloproteinase (MMP)-2 and -9 expression in bronchoalveolar lavage fluid (BALF) of horses with chronic inflammation of the lungs (ie, lower airway inflammation [LAI]). ANIMALS 29 horses with signs of chronic respiratory tract disease, which were classified as the LAI (n = 17) and LAI with respiratory distress (RDLAI [12]) groups, and 15 control horses. PROCEDURES BALF, tracheal aspirate, and blood samples were obtained, and EMMPRIN expression was determined from BALF cells and RBCs by use of western blotting. Activities of MMP-2 and -9 were determined with zymography. RESULTS Expression of EMMPRIN protein was identified in BALF cells of all horses. Expression of EMMPRIN protein was highest for the RDLAI group and was correlated with MMP-2 and -9 protein expression, MMP-9 gelatinolytic activity, and airway neutrophilia. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that EMMPRIN was involved in the pathophysiologic processes of asthma in horses. However, additional studies of horses and other species are warranted to elucidate the regulation of EMMPRIN expression in asthmatic lungs.
目的 检测细胞外基质金属蛋白酶诱导剂（EMMPRIN）在马肺中的表达情况，以及其与患有慢性肺部炎症（即下呼吸道炎症[LAI]）的马支气管肺泡灌洗液（BALF）中基质金属蛋白酶（MMP）-2和-9表达的相关性。动物 29匹有慢性呼吸道疾病体征的马，分为LAI组（n = 17）和伴有呼吸窘迫的LAI（RDLAI[12]）组，以及15匹对照马。方法 获取BALF、气管吸出物和血液样本，通过蛋白质印迹法测定BALF细胞和红细胞中的EMMPRIN表达。用酶谱法测定MMP-2和-9的活性。结果 在所有马的BALF细胞中均鉴定出EMMPRIN蛋白表达。RDLAI组的EMMPRIN蛋白表达最高，且与MMP-2和-9蛋白表达、MMP-9明胶酶活性及气道嗜中性粒细胞增多相关。结论及临床意义 结果表明EMMPRIN参与了马哮喘的病理生理过程。然而，有必要对马和其他物种进行更多研究，以阐明哮喘肺中EMMPRIN表达的调控机制。
