Sawicka-Zugaj W, Chabuz W, Litwińczuk Z, Kasprzak-Filipek K
Department of Breeding and Protection of Genetic Resources of Cattle, University of Life Sciences in Lublin, Lublin, Poland.
Department of Commodity Science and Processing of Raw Animal Materials, University of Life Sciences in Lublin, Lublin, Poland.
Reprod Domest Anim. 2018 Feb;53(1):157-162. doi: 10.1111/rda.13085. Epub 2017 Oct 27.
The aim of the study was to evaluate genetic variation, reproductive performance and the degree of relationship of White-Backed bulls entered in the breed registry and approved for breeding. The study included 32 bulls of the White-Backed (WB) breed with an entry in the breed registry and used for breeding in the years 2003-2015. Eleven microsatellite DNA sequences (TGLA222, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225 and BM1824) were used to analyse variation in the WB bulls. The bulls most often used for breeding were Chilon (1,073 doses), Mak (939 doses) and Jaguar (858 doses). The bulls Mak, Chilon and Jak had the greatest influence on the active population of White-Backed cattle, with the most daughters. In the analysed population of White-Backed bulls, a total of 79 different alleles were identified, with a mean 7.27 per locus. The analysed pool of microsatellite loci was characterized by high values for PIC, H and H (>0.6), and the entire population was in genetic equilibrium. The estimated level of inbreeding within the population ranged from -0.2277 (ETH225) to 0.0775 (SPS115), with a mean value of -0.0587.
本研究的目的是评估列入品种登记册并被批准用于繁殖的白背公牛的遗传变异、繁殖性能以及亲缘关系程度。该研究包括32头列入白背（WB）品种登记册并于2003年至2015年用于繁殖的公牛。使用11个微卫星DNA序列（TGLA222、BM2113、TGLA53、ETH10、SPS115、TGLA126、TGLA122、INRA23、ETH3、ETH225和BM1824）分析白背公牛的变异情况。最常用于繁殖的公牛是奇隆（1073剂）、马克（939剂）和捷豹（858剂）。公牛马克、奇隆和雅克对白背牛的有效种群影响最大，拥有的女儿最多。在分析的白背公牛群体中，共鉴定出79个不同的等位基因，每个位点平均为7.27个。所分析的微卫星基因座库的特征是PIC、H和H值较高（>0.6），且整个群体处于遗传平衡状态。群体内估计的近亲繁殖水平范围为-0.2277（ETH225）至0.0775（SPS115），平均值为-0.0587。
