Kudryashov Nikita V, Kalinina Tatiana S, Shimshirt Alexander A, Korolev Anton O, Volkova Anna V, Voronina Tatiana A
Department of Psychopharmacology, Federal State Budgetary Institution 'Research Zakusov Institute of Pharmacology'.
Department of Pharmacology of Medicine Faculty, Federal State Autonomous Educational Institution of Higher Education, I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation, Sechenov University, Moscow, Russian Federation.
Behav Pharmacol. 2018 Jun;29(4):375-378. doi: 10.1097/FBP.0000000000000359.
The antidepressant-like effect of fluoxetine (20 mg/kg i.p.) has been assessed using the forced swimming test (FST) in IRC (CD-1) mice exposed or not to a pretest session of different duration (5 or 20 min). The influence of the mitochondrial translocator protein (TSPO) activity on the antidepressant-like effect of fluoxetine (20 mg/kg i.p.) in the FST was also studied. The antidepressant-like effect of fluoxetine was observed only in mice subjected to a 5-min pretest session 24 h before the FST. The TSPO antagonist PK11195 [1-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)-3-isoquinolinecarboxamide; 1 or 3 mg/kg i.p.] inhibited the antidepressant activity of fluoxetine in the FST. In the present study, fluoxetine or PK11195 was administered for a short duration. We suppose that the functional activity of TSPO may depend on a pretest session and that using this procedure is necessary to detect antidepressant activity of fluoxetine-like drugs.
已使用强迫游泳试验（FST）在暴露或未暴露于不同时长（5或20分钟）预试验的IRC（CD-1）小鼠中评估了氟西汀（20毫克/千克腹腔注射）的抗抑郁样作用。还研究了线粒体转位蛋白（TSPO）活性对氟西汀（20毫克/千克腹腔注射）在FST中的抗抑郁样作用的影响。仅在FST前24小时接受5分钟预试验的小鼠中观察到氟西汀的抗抑郁样作用。TSPO拮抗剂PK11195 [1-(2-氯苯基)-N-甲基-N-(1-甲基丙基)-3-异喹啉甲酰胺；1或3毫克/千克腹腔注射]抑制了氟西汀在FST中的抗抑郁活性。在本研究中，氟西汀或PK11195给药时间较短。我们推测TSPO的功能活性可能取决于预试验，并且使用该程序对于检测氟西汀样药物的抗抑郁活性是必要的。
