在蜥蜴眼斑沙蜥发育中的胸腺中检测 Thy-1+ 细胞。
Detection of Thy-1+ cells in the developing thymus of the lizard, Chalcides ocellatus.
作者信息
el Deeb S, Saad A H, Zapata A
机构信息
Zoology Department, Faculty of Science, Cairo University, Egypt.
出版信息
Thymus. 1988;12(1):3-9.
The ontogenic appearance of Thy-1+ cells was studied in the thymus of the lizard, Chalcides ocellatus, by indirect immunofluorescence using a rabbit antibrain antiserum produced against surface determinants shared by both the thymus and the brain lizards. Thymic Thy-1+ cells were first detected at stage 37. They increased rapidly reaching adult numbers by stage 41. Apparently Thy-1+ cells differentiate from lymphoid precursors arriving at the thymus analage before stage 37. The results are compared to those found during the differentiation of Thy-1+ thymocyte in murine thymus and agree with former reports on the thymic development in Chalcides using rabbit antisera against adult thymocyte marker(s).
利用针对蜥蜴胸腺和脑共有的表面决定簇产生的兔抗脑抗血清，通过间接免疫荧光法研究了蜥蜴（眼斑沙蜥）胸腺中Thy-1+细胞的个体发生情况。胸腺Thy-1+细胞在第37阶段首次被检测到。它们迅速增加，到第41阶段达到成年数量。显然，Thy-1+细胞是由在第37阶段之前到达胸腺原基的淋巴样前体细胞分化而来的。将这些结果与在小鼠胸腺中Thy-1+胸腺细胞分化过程中发现的结果进行了比较，并且与之前关于使用针对成年胸腺细胞标记物的兔抗血清对眼斑沙蜥胸腺发育的报道一致。
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