Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN 55455, USA.
Lab Chip. 2017 Dec 19;18(1):27-40. doi: 10.1039/c7lc01105a.
Micro free-flow electrophoresis (μFFE) is a continuous separation technique in which analytes are streamed through a perpendicularly applied electric field in a planar separation channel. Analyte streams are deflected laterally based on their electrophoretic mobilities as they flow through the separation channel. A number of μFFE separation modes have been demonstrated, including free zone (FZ), micellar electrokinetic chromatography (MEKC), isoelectric focusing (IEF) and isotachophoresis (ITP). Approximately 60 articles have been published since the first μFFE device was fabricated in 1994. We anticipate that recent advances in device design, detection, and fabrication, will allow μFFE to be applied to a much wider range of applications. Applications particularly well suited for μFFE analysis include continuous, real time monitoring and microscale purifications.
微流自由电泳（μFFE）是一种连续分离技术，其中分析物在平面分离通道中通过垂直施加的电场被输送。分析物流在通过分离通道时根据其电泳迁移率横向偏转。已经证明了许多 μFFE 分离模式，包括自由区（FZ）、胶束电动色谱（MEKC）、等电聚焦（IEF）和等速电泳（ITP）。自 1994 年制造出第一个 μFFE 装置以来，已经发表了大约 60 篇文章。我们预计，在设备设计、检测和制造方面的最新进展，将使 μFFE 能够应用于更广泛的应用领域。特别适合 μFFE 分析的应用包括连续、实时监测和微尺度纯化。
