Liphardt Anna-Maria, Mündermann Annegret, Andriacchi Thomas P, Achtzehn Silvia, Heer Martina, Mester Joachim
German Sport University Cologne (DSHS Köln), Training Science and Sport Informatics, Köln, Germany.
German Sport University Cologne (DSHS Köln), Biomechanics and Orthopaedics, Köln, Germany.
J Orthop Res. 2018 May;36(5):1465-1471. doi: 10.1002/jor.23786. Epub 2017 Nov 17.
The objective of the study was to test the hypothesis that serum levels of cartilage oligomeric matrix protein (COMP) would decrease and serum levels of tumor-necrosis factor alpha (TNF-α) and selected matrix metalloproteinases (MMPs) would increase in response to bed rest (BR) and that these changes are unaffected by the intake of potassium bicarbonate or whey protein. Seven and nine healthy male subjects participated in two 21-day 6° head down tilt crossover BR-studies with nutrition interventions. Serum samples were taken before, during, and after BR and biomarker concentrations were measured using commercial enzyme-linked immunosorbent assays. MMP-3 during BR was significantly lower than at baseline (reduction greater 20%; p < 0.001). MMP-3 increased significantly from 14 to 21 days of BR (+7%; p = 0.049). COMP during BR was significantly lower than at baseline (reduction greater 20%; p < 0.001). MMP-3 and COMP returned to baseline within 1 day after BR. MMP-9 on day 3 of BR was significantly lower than at baseline (-31%; p < 0.033) and on days 3, 5, and 14 of BR significantly lower than at the end of and after BR (reduction greater 35%; p < 0.030). The nutritional countermeasures did not affect these results. The observed changes in cartilage biomarkers may be caused by altered cartilage metabolism in response to the lack of mechanical stimulus during BR and inflammatory biomarkers may play a role in changes in biomarker levels.
Immobilization independently from injury can cause altered cartilage biomarker concentration. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1465-1471, 2018.
本研究的目的是检验以下假设：卧床休息（BR）会导致血清软骨寡聚基质蛋白（COMP）水平降低，血清肿瘤坏死因子α（TNF-α）和选定的基质金属蛋白酶（MMPs）水平升高，且这些变化不受碳酸氢钾或乳清蛋白摄入的影响。7名和9名健康男性受试者参与了两项为期21天的6°头低位倾斜交叉BR研究，并进行了营养干预。在BR前、期间和之后采集血清样本，并使用商业酶联免疫吸附测定法测量生物标志物浓度。BR期间的MMP-3显著低于基线水平（降低超过20%；p < 0.001）。BR第14至21天，MMP-3显著升高（升高7%；p = 0.049）。BR期间的COMP显著低于基线水平（降低超过20%；p < 0.001）。BR后1天内，MMP-3和COMP恢复到基线水平。BR第3天的MMP-9显著低于基线水平（降低31%；p < 0.033），且在BR第3、5和14天显著低于BR结束时和之后的水平（降低超过35%；p < 0.030）。营养对策并未影响这些结果。观察到的软骨生物标志物变化可能是由于BR期间缺乏机械刺激导致软骨代谢改变引起的，炎症生物标志物可能在生物标志物水平变化中起作用。
与损伤无关的固定可导致软骨生物标志物浓度改变。© 2017骨科研究协会。由Wiley Periodicals, Inc.出版。《矫形外科研究杂志》36:1465 - 1471, 2018年。
