Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of the Philippines, Los Baños, College, Laguna, 4031 Philippines.
Poultry Products Quality Control, Ltd. 125-7 Daiwa Dakeonsen, Nihonmatsu-shi, Fukushima-Prefecture, Japan.
Poult Sci. 2018 Jan 1;97(1):24-29. doi: 10.3382/ps/pex254.
From a total of 72 commercial layer and pullet farms that were monitored in the eastern Japan area, 4 farms had mild to severe respiratory disease accompanied by decreased feed intake and drop in egg production. Microbiological analysis showed that 3 of the 4 farms, particularly from Fukushima, Tochigi, and Ibaraki prefectures, were positive for Ornithobacterum rhinotracheale (ORT). Out of 65 birds examined, ORT was isolated in 21 birds (32.31%). All isolates were Gram-negative pleomorphic rods with a colony size of 0.05 mm, translucent with grayish coloration, and with butyric smell after 48 h of incubation in 10% chicken blood agar at 37°C under microaerophilic conditions. All isolates reacted positively in the p-nitrophenyl-β-d-galactopyranoside test within 3 h and were positive in cytochrome oxidase tests with an API 20NE identification system biocode of 0-0-2-0-0-0-4. An agar gel precipitation test showed that all isolates were serotype-A. All strains were positive in PCR by yielding a 784 bp amplicon of the 16S rRNA gene. All strains were resistant to amikacin, colistin, gentamicin, kanamycin, neomycin, polymyxin b, streptomycin, and sulfamethoxazole trimethoprim and susceptible to amoxicillin clavulanic acid, ampicillin, doxycycline, spectinomycin, and tetracycline. This study is the first characterization of ORT from commercial layer chickens in eastern Japan.
在日本东部地区监测的 72 个商业蛋鸡和育成鸡养殖场中，有 4 个场出现了轻度至重度呼吸道疾病，伴有采食量下降和产蛋量下降。微生物分析显示，4 个养殖场中有 3 个（来自福岛、栃木和茨城县）为鸟疫杆菌（ORT）阳性。在检查的 65 只禽鸟中，有 21 只（32.31%）分离出了 ORT。所有分离株均为革兰氏阴性多形杆菌，菌落大小为 0.05mm，半透明，呈灰白色，在 37°C 微需氧条件下，在 10%鸡血液琼脂中孵育 48 小时后，有丁酸气味。所有分离株在 p-硝基苯-β-d-半乳糖吡喃糖苷试验中在 3 小时内均呈阳性，在 API 20NE 鉴定系统生物码 0-0-2-0-0-0-4 的细胞色素氧化酶试验中呈阳性。琼脂凝胶沉淀试验显示，所有分离株均为 A 血清型。所有菌株在 16S rRNA 基因的 PCR 中均产生 784bp 的扩增子。所有菌株均对阿米卡星、黏菌素、庆大霉素、卡那霉素、新霉素、多粘菌素 B、链霉素和磺胺甲噁唑-甲氧苄啶耐药，对阿莫西林克拉维酸、氨苄西林、多西环素、壮观霉素和四环素敏感。本研究首次对日本东部商业蛋鸡中的 ORT 进行了特征描述。
