Luo Lanxin, Cui Yan, Zhang Shuting, Li Lingxi, Suo Hao, Sun Baoshan
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 110016, Shenyang, China.
School of Functional Food and Wine, Shenyang Pharmaceutical University, 110016, Shenyang, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Nov 15;1068-1069:201-209. doi: 10.1016/j.jchromb.2017.10.031. Epub 2017 Oct 16.
Vidal Blanc grape (Vitis vinifera cv.) is the predominant white grape variety used for the production of icewine in China's Liaoning province. In this paper, the development and validation of the method by ultrahigh-performance liquid chromatography-tandem mass spectrometry has been performed for determination of the detailed phenolic composition in the skin, seed and stem of Vidal grapes. The validation of the method was realized by calculating the linearity, repeatability, precision, stability and the limits of detection (LOD) and quantification (LOQ) of standard solutions. All the curves exhibited good linearity (r>0.9997) and the LOD and LOQ were in the range of 0.002-0.025 and 0.006-0.086μg/ml, respectively. Good repeatability (RSD<4.3%) and stability (RSD<3.7%) were also found. Results confirmed that the developed method was more effective and sensitive for simultaneous determination of the major phenolic compounds in Vidal grape pomace. The optimized and validated method of ultrahigh-performance liquid chromatography tandem two complementary techniques, fourier transform ion cyclotron resonance mass spectrometry and triple-quadrupole mass spectrometry, allowed to identify and quantify up to 35 phenolic compounds in Vidal grape pomace, which has, as far as we know, been reported this grapevine variety for the first time. Seeds, skins and stems exhibited different qualitative and quantitative phenolic profiles. These results provided useful information for recovery of phenolic antioxidants from different parts of icewine pomace.
维代尔白葡萄（Vitis vinifera cv.）是中国辽宁省用于生产冰酒的主要白葡萄品种。本文采用超高效液相色谱-串联质谱法对维代尔葡萄的果皮、种子和茎中的详细酚类成分进行了测定方法的开发与验证。通过计算标准溶液的线性、重复性、精密度、稳定性以及检测限（LOD）和定量限（LOQ）来实现方法的验证。所有曲线均呈现良好的线性（r>0.9997），检测限和定量限分别在0.002 - 0.025和0.006 - 0.086μg/ml范围内。还发现了良好的重复性（RSD<4.3%）和稳定性（RSD<3.7%）。结果证实，所开发的方法对于同时测定维代尔葡萄果渣中的主要酚类化合物更有效且灵敏。超高效液相色谱串联傅里叶变换离子回旋共振质谱和三重四极杆质谱这两种互补技术的优化和验证方法，能够鉴定和定量维代尔葡萄果渣中多达35种酚类化合物，据我们所知，这是首次针对该葡萄品种进行报道。种子、果皮和茎呈现出不同的酚类定性和定量特征。这些结果为从冰酒果渣的不同部位回收酚类抗氧化剂提供了有用信息。
