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采用高效液相色谱-二极管阵列检测-电喷雾串联质谱法分析 Bordô 葡萄(Vitis labrusca)可食用部分(果肉和果皮)的酚类成分。

Phenolic composition of the edible parts (flesh and skin) of Bordô grape (Vitis labrusca) using HPLC-DAD-ESI-MS/MS.

机构信息

Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, Rua Cristovão Colombo, 2265 Jardim Nazareth, CEP 15054-000 São José do Rio Preto, SP, Brazil.

出版信息

J Agric Food Chem. 2011 Dec 28;59(24):13136-46. doi: 10.1021/jf203679n. Epub 2011 Dec 5.

DOI:10.1021/jf203679n
PMID:22112247
Abstract

The aim of this study was the detailed characterization of the phenolic composition and the determination of the antioxidant activity of the Bordô grape (Vitis labrusca) cultivated in South Brazil. The edible parts of Bordô grapes (flesh and skin) contained 1130 mg/kg of total phenolic compounds (as gallic acid), mainly located in the skins. Anthocyanin content in the skins was high, largely as 3,5-diglucosides (1359 mg/kg, as malvidin 3,5-diglucoside). Total flavonols accounted for 154 μmol/kg, mainly located in the skins and with myricetin 3-glucoside as the principal flavonol in both grape parts. Very low amounts of flavan-3-ol monomers and dimers and low amounts of polymeric proanthocyanidins, with a composition similar to that reported for V. vinifera grape varieties, were found in Bordô grape skins. Hydroxycinnamic acid derivatives mainly derived from caffeic acid and were found in the skins in high amounts, ten times higher than in the flesh (total amount: 483 μmol/kg). Finally, the Bordô grape cultivar can be considered a high resveratrol producer (10.91 mg/kg) and also exhibited a high value of total antioxidant capacity (37.6 ± 1.0 mmol/kg, as Trolox).

摘要

本研究的目的是详细描述南里奥格兰德州种植的 Bordô 葡萄(Vitis labrusca)的酚类成分组成,并测定其抗氧化活性。Bordô 葡萄的可食用部分(果皮和果肉)含有 1130mg/kg 的总酚类化合物(以没食子酸计),主要存在于果皮中。果皮中的花色苷含量很高,主要为 3,5-二葡萄糖苷(1359mg/kg,以矢车菊素 3,5-二葡萄糖苷计)。总类黄酮含量为 154μmol/kg,主要存在于果皮中,且在葡萄的两个部位中,主要的类黄酮均为杨梅素 3-葡萄糖苷。Bordô 葡萄果皮中仅检测到少量的黄烷-3-醇单体和二聚体,以及少量组成与 V. vinifera 葡萄品种相似的聚合原花青素。另外,Bordô 葡萄果皮中还含有大量的羟基肉桂酸衍生物,主要来源于咖啡酸,其含量是果肉中的 10 倍(总量:483μmol/kg)。最后,Bordô 葡萄品种可以被认为是一种高白藜芦醇的生产者(10.91mg/kg),其总抗氧化能力值也很高(37.6±1.0mmol/kg,以 Trolox 计)。

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