Regional Institute for Applied Scientific Research (IRICA), University of Castilla-La Mancha, Ciudad Real, Spain.
Higher Technical School of Agronomic Engineering, University of Castilla-La Mancha, Ciudad Real, Spain.
J Sci Food Agric. 2022 Jan 30;102(2):813-822. doi: 10.1002/jsfa.11416. Epub 2021 Jul 19.
'Karaerik' is a novel table grape (Vitis vinifera L.) native to Turkey and widely cultivated in areas bordering the city of Erzincan. Because of the demonstrated beneficial effects on human health of the grape phenolic composition, the aim of this work was to conduct a detailed profiling of non-anthocyanin phenolic fractions from different grape tissues of the 'Karaerik' table grape. Both qualitative and quantitative characterization of phenolic compounds were achieved using high-performance liquid chromatography-diode array detection-electrospray ionization-tandem mass spectrometry. Total phenolic content and oxygen radical absorbance capacity were also determined to evaluate the antioxidant properties of this table grape.
A high number of non-anthocyanin phenolic compounds was identified in 'Karaerik' table grape skins and seeds, including 11 flavonols, six hydroxycinnamic acid derivatives, two stilbenes, several monomeric and dimeric flavan-3-ols and proanthocyanidins. Quercetin-type derivatives dominated the flavonol profile of grape skins, followed by myricetin type. Tartaric acid esters of three acids (caffeic, coumaric and ferulic acids) were the main hydroxycinnamic acid derivatives in this cultivar. Qualitative and quantitative differences were observed in flavan-3-ol composition among the grape tissues. Proanthocyanidins were the most abundant class of phenolic compounds in 'Karaerik' grapes, being mainly located in seeds. Higher antioxidant capacity values were determined in grape seeds, in correlation with the total phenolic content.
These results provide useful information for a better understanding of phenolic antioxidants from the 'Karaerik' table grape and will contribute to promoting the varietal identity and health-related properties of this fruit. © 2021 Society of Chemical Industry.
“卡拉埃雷克”是一种原产于土耳其的新型鲜食葡萄（Vitis vinifera L.），在埃雷利省周边地区广泛种植。由于葡萄酚类成分对人体健康具有有益作用，因此本研究旨在对“卡拉埃雷克”鲜食葡萄不同组织中非花色苷酚类成分进行详细分析。采用高效液相色谱-二极管阵列检测-电喷雾电离-串联质谱法对酚类化合物进行定性和定量分析，同时还测定了总酚含量和氧自由基吸收能力，以评估该鲜食葡萄的抗氧化特性。
在“卡拉埃雷克”鲜食葡萄皮和种子中鉴定出大量非花色苷酚类化合物，包括 11 种黄酮醇、6 种羟基肉桂酸衍生物、2 种芪类、几种单体和二聚黄烷-3-醇和原花青素。葡萄皮中黄酮醇图谱以槲皮素型衍生物为主，其次是杨梅素型。该品种中主要的羟基肉桂酸衍生物是三种酸（咖啡酸、香豆酸和阿魏酸）的酒石酸酯。在不同的葡萄组织中观察到黄烷-3-醇组成存在定性和定量差异。原花青素是“卡拉埃雷克”葡萄中含量最丰富的酚类化合物，主要存在于种子中。葡萄种子的抗氧化能力值较高，与总酚含量呈正相关。
这些结果为更好地了解“卡拉埃雷克”鲜食葡萄中的酚类抗氧化剂提供了有用的信息，并将有助于促进该品种的品种特性和与健康相关的特性。© 2021 英国化学学会。
