Effect of troxerutin and methionine on spin trapping of free oxy-radicals.

The cardioprotective agents troxerutin and methionine are radical scavengers and compete with the DMPO adduct formation of .OH generated by the Fenton reaction. The concentration of trapped .O2- generated by the xanthine oxidase/hypoxanthine reaction is lowered in the presence of troxerutin. The decay of DMPO-OH is decreased by troxerutin compared to the control. In the presence of methionine a carbon-centered radical is produced. The investigations support the opinion that the scavenging of oxygen derived free radicals is of importance for the cardioprotective action of these agents.