Nomura Yudai, Takamatsu Toshihiro, Kawano Hiroaki, Miyahara Hidekazu, Okino Akitoshi, Yoshida Masaru, Azuma Takeshi
Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.
Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan; Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, Japan.
J Surg Res. 2017 Nov;219:302-309. doi: 10.1016/j.jss.2017.06.055.
Nonthermal atmospheric pressure plasma (NTAPP) has recently received attention as a novel tool in medicine. It is thought that plasma components yield plasma effects such as sterilization, blood coagulation, and wound healing. These effects are produced without thermal damage. We investigated the blood coagulation effect of NTAPP by using a multigas plasma jet.
Multigas plasma jets can generate NTAPP by several gas species. In this study, argon, oxygen, helium, nitrogen, mock air, and carbon dioxide were used to generate NTAPP, and blood coagulation times were compared with each plasma-treated sample. The NTAPP blood coagulation effects on whole blood with four different anticoagulants were investigated. In addition, in this study, the effects of plasma treatment on porcine tissues and organs were investigated as in vivo experiment.
A tendency to coagulate later with argon gas plasma than others was shown. There were no significant differences between oxygen, helium, nitrogen, mock air, and carbon dioxide. Whole blood with each anticoagulant demonstrated fast coagulation by NTAPP treatment. Fast control of the bleeding lesions on porcine stomach and liver by plasma treatment was observed, and no tissue damage due to the plasma treatment was detected by optical microscope.
These experiments suggest the potential of various gas NTAPPs as a novel medical device to control bleeding lesions.
非热大气压等离子体（NTAPP）最近作为医学领域的一种新型工具受到关注。人们认为等离子体成分会产生诸如杀菌、凝血和伤口愈合等等离子体效应。这些效应是在无热损伤的情况下产生的。我们使用多气体等离子体射流研究了NTAPP的凝血效应。
多气体等离子体射流可通过多种气体产生NTAPP。在本研究中，使用氩气、氧气、氦气、氮气、模拟空气和二氧化碳来产生NTAPP，并比较每个经等离子体处理样品的凝血时间。研究了NTAPP对含有四种不同抗凝剂的全血的凝血效应。此外，在本研究中，还进行了体内实验，研究了等离子体处理对猪组织和器官的影响。
结果显示，氩气等离子体处理后的凝血倾向比其他气体处理的要晚。氧气、氦气、氮气、模拟空气和二氧化碳之间没有显著差异。经NTAPP处理后，含有每种抗凝剂的全血均表现出快速凝血。观察到等离子体处理能快速控制猪胃和肝脏上的出血损伤，光学显微镜未检测到因等离子体处理造成的组织损伤。
这些实验表明，各种气体的NTAPP作为一种控制出血损伤的新型医疗设备具有潜力。
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