Plasma Bioscience Research Center, Applied Plasma Medicine Center, Kwangwoon University, Seoul 01897, Korea.
Biomedical Research Institute, Seoul National University Hospital, Seoul 03080, Korea.
Cells. 2021 Oct 13;10(10):2728. doi: 10.3390/cells10102728.
Pelvic organ prolapse (POP) is a chronic disorder that affects quality of life in women. Several POP treatments may be accompanied by abrasion, constant infection, and severe pain. Therefore, new treatment methods and improvements in current treatments for POP are required. Non-thermal atmospheric-pressure plasma is a rising biomedical therapy that generates a mixed cocktail of reactive species by different mechanisms. In this study, we applied a cylinder-type dielectric barrier discharge plasma device to create a plasma-treated liquid (PTL). The PTL was added to primary cultured human uterosacral ligament fibroblast (hUSLF) cells from POP patients at each stage. Surprisingly, treatment with diluted PTL increased hUSLF cell viability but decreased ovarian cancer cell viability. PTL also decreased cell apoptosis in hUSLF cells but increased it in SKOV3 cells. Our results suggest that PTL protects hUSLF cells from cell apoptosis by controlling the p53 pathway and improves cell viability, implying that PTL is a promising application for POP therapy.
盆腔器官脱垂（POP）是一种影响女性生活质量的慢性疾病。一些 POP 治疗方法可能伴随着磨损、持续感染和严重疼痛。因此，需要新的治疗方法和改进目前的 POP 治疗方法。非热常压等离子体是一种新兴的生物医学治疗方法，通过不同的机制产生混合的反应物种。在这项研究中，我们应用了一种圆柱形介电阻挡放电等离子体装置来创建等离子体处理液（PTL）。将 PTL 添加到每个阶段来自 POP 患者的原代培养人子宫骶骨韧带成纤维细胞（hUSLF）中。令人惊讶的是，稀释的 PTL 处理增加了 hUSLF 细胞活力，但降低了卵巢癌细胞活力。PTL 还降低了 hUSLF 细胞的细胞凋亡，但增加了 SKOV3 细胞的凋亡。我们的结果表明，PTL 通过控制 p53 通路保护 hUSLF 细胞免受细胞凋亡，并提高细胞活力，这表明 PTL 是一种有前途的 POP 治疗应用。
