Keylaboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
Fungal Genet Biol. 2017 Dec;109:46-52. doi: 10.1016/j.fgb.2017.10.007. Epub 2017 Oct 25.
Phospholipase D (PLD) is an important phospholipid hydrolase that plays critical roles in various biological processes in eukaryotic cells. However, little is known about its functions in plant pathogenic fungi. In this study, we identified three FgPLD genes in Fusarium graminearum that are homologous to the Saccharomyces cerevisiae Spo14 gene. We constructed deletion mutants of all three FgPLD genes using homologous recombination. Deletion of FgPLD1 (Δpld1), but not FgPLD2 or FgPLD3, affected hyphal growth, conidiation, and perithecium formation. The Δpld1 mutant showed reduced deoxynivalenol (DON) production and virulence in flowering wheat heads and corn silks. Furthermore, three FgPLD proteins have the same subcellular localization and localize to the cytoplasm in F. graminearum. Taken together, these results indicate that FgPLD1, but not FgPLD2 or FgPLD3, is important for hyphal growth, sexual or asexual reproduction, and plant infection.
磷脂酶 D（PLD）是一种重要的磷脂水解酶，在真核细胞的各种生物过程中发挥着关键作用。然而，关于其在植物病原真菌中的功能知之甚少。在本研究中，我们在禾谷镰刀菌中鉴定了三个与酿酒酵母 Spo14 基因同源的 FgPLD 基因。我们使用同源重组构建了这三个 FgPLD 基因的缺失突变体。缺失 FgPLD1（Δpld1），而不是 FgPLD2 或 FgPLD3，影响菌丝生长、分生孢子形成和产囊体形成。Δpld1 突变体在开花小麦穗和玉米丝中产生的脱氧雪腐镰刀菌烯醇（DON）减少，毒力降低。此外，三个 FgPLD 蛋白具有相同的亚细胞定位，在禾谷镰刀菌中定位于细胞质。综上所述，这些结果表明 FgPLD1，而不是 FgPLD2 或 FgPLD3，对菌丝生长、有性或无性繁殖以及植物感染很重要。
