Zhao Chenming, Yang Xiaoyue, Jiang Wenqiang, Zhang Guifen, Ma Dongfang
Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River, College of Agriculture, Yangtze University, Jingzhou 434025, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
J Fungi (Basel). 2024 Mar 11;10(3):208. doi: 10.3390/jof10030208.
GDP-mannose transporters (GMTs) have been implicated in the virulence of some important pathogenic fungi, and guanosine diphosphate (GDP) mannose transporters transport GDP-mannose from the cytosol to the Golgi lumen prior to mannosylation, where mannose attaches to the modified protein. GMTs could be potential targets for new antifungal drugs, as disruption of any step in GDP-mannose biosynthesis can affect fungal viability, growth, or virulence. To date, the GDP-mannose transporter has been extensively studied in yeast, but its biological function in fungi, particularly , is still unclear. In this experimental study, the role of the GDP-mannose transporter in was investigated by analysing the gene. and were blastp-derived from their protein sequences and proved to be their functional homologues. The mutant and complementary strains of , and genes were generated and used to evaluate the effect of the two GMTs genes on mycelial growth, asexual reproduction, sexual reproduction, cell wall sensitivity, glyphosate synthesis and drug susceptibility. Only in the and mutants was the rate of mycelial growth slowed, conidium production increased, sexual reproduction impaired, cell wall sensitivity increased, glycemic content decreased, and drug sensitivity reduced. The results of the pathogenicity assessment of GMTs showed that only affects the patogenicity of . At the same time, the effect of GMTs on the ability of rhinoceros to synthesise DON toxins was investigated and the results showed that the ability of Δ and Δ mutants to produce the DON toxin was significantly reduced, and the expression of toxin-related genes was also reduced.
GDP-甘露糖转运蛋白（GMTs）与一些重要致病真菌的毒力有关，鸟苷二磷酸（GDP）甘露糖转运蛋白在甘露糖基化之前将GDP-甘露糖从细胞质转运到高尔基体腔中，在高尔基体腔中甘露糖附着于修饰的蛋白质上。GMTs可能是新型抗真菌药物的潜在靶点，因为GDP-甘露糖生物合成中任何步骤的破坏都可能影响真菌的生存能力、生长或毒力。迄今为止，GDP-甘露糖转运蛋白已在酵母中得到广泛研究，但其在真菌中的生物学功能，尤其是在[具体真菌名称未给出]中的功能仍不清楚。在本实验研究中，通过分析[具体基因名称未给出]基因来研究GDP-甘露糖转运蛋白在[具体真菌名称未给出]中的作用。[具体基因名称未给出]和[具体基因名称未给出]是通过对它们的[具体蛋白名称未给出]蛋白序列进行blastp比对得到的，并被证明是它们的功能同源物。构建了[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]基因的突变体和互补菌株，用于评估这两个GMTs基因对菌丝生长、无性繁殖、有性繁殖、细胞壁敏感性、草甘膦合成和药物敏感性的影响。仅在[具体基因名称未给出]和[具体基因名称未给出]突变体中，菌丝生长速率减慢、分生孢子产量增加、有性繁殖受损、细胞壁敏感性增加、血糖含量降低且药物敏感性降低。GMTs致病性评估结果表明，只有[具体基因名称未给出]影响[具体真菌名称未给出]的致病性。同时，研究了GMTs对[具体真菌名称未给出]合成脱氧雪腐镰刀菌烯醇（DON）毒素能力的影响，结果表明Δ[具体基因名称未给出]和Δ[具体基因名称未给出]突变体产生DON毒素的能力显著降低，且毒素相关基因的表达也降低。
