Skrzynska Arleta Krystyna, Martos-Sitcha Juan Antonio, Martínez-Rodríguez Gonzalo, Mancera Juan Miguel
Department of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cádiz, 11519 Puerto Real, Cádiz, Spain.
Instituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), 11519 Puerto Real, Cádiz, Spain; Nutrigenomics and Fish Growth Endocrinology Group, Institute of Aquaculture Torre de la Sal, Consejo Superior de Investigaciones Científicas (IATS-CSIC), Ribera de Cabanes, E-12595 Castellón, Spain.
Comp Biochem Physiol A Mol Integr Physiol. 2018 Jan;215:35-44. doi: 10.1016/j.cbpa.2017.10.012. Epub 2017 Oct 24.
The influence of chronic stress, induced by food deprivation (FD) and/or high stocking density (HSD), was assessed on stress, vasotocinergic and isotocinergic pathways of the gilthead sea bream (Sparus aurata). Fish were randomly assigned to one of the following treatments: (1) fed at low stocking density (LSD-F; 5kg·m); (2) fed at high stocking density (HSD-F, 40kg·m); (3) food-deprived at LSD (LSD-FD); and (4) food-deprived at HSD (HSD-FD). After 21days, samples from plasma, liver, hypothalamus, pituitary and head-kidney were collected. Both stressors (FD and HSD) induced a chronic stress situation, as indicated by the elevated cortisol levels, the enhancement in corticotrophin releasing hormone (crh) expression and the down-regulation in corticotrophin releasing hormone binding protein (crhbp) expression. Changes in plasma and liver metabolites confirmed a metabolic adjustment to cope with energy demand imposed by stressors. Changes in avt and it gene expression, as well as in their specific receptors (avtrv1a, avtrv2 and itr) at central (hypothalamus and pituitary) and peripheral (liver and head-kidney) levels, showed that vasotocinergic and isotocinergic pathways are involved in physiological changes induced by FD or HSD, suggesting that different stressors are handled through different stress pathways in S. aurata.
评估了由食物剥夺（FD）和/或高养殖密度（HSD）诱导的慢性应激对金头鲷（Sparus aurata）应激、血管紧张素能和异速加压素能途径的影响。将鱼随机分配到以下处理组之一：（1）低密度养殖投喂（LSD-F；5kg·m）；（2）高密度养殖投喂（HSD-F，40kg·m）；（3）低密度养殖食物剥夺（LSD-FD）；（4）高密度养殖食物剥夺（HSD-FD）。21天后，收集血浆、肝脏、下丘脑、垂体和头肾的样本。两种应激源（FD和HSD）均诱导了慢性应激状态，这表现为皮质醇水平升高、促肾上腺皮质激素释放激素（crh）表达增强以及促肾上腺皮质激素释放激素结合蛋白（crhbp）表达下调。血浆和肝脏代谢物的变化证实了为应对应激源施加的能量需求而进行的代谢调整。血管紧张素（avt）和异速加压素（it）基因及其在中枢（下丘脑和垂体）和外周（肝脏和头肾）水平的特异性受体（avtrv1a、avtrv2和itr）的表达变化表明，血管紧张素能和异速加压素能途径参与了由FD或HSD诱导的生理变化，这表明在金头鲷中不同的应激源通过不同的应激途径来处理。
