Tejero Rioseras Alberto, Garcia Gomez Diego, Ebert Birgitta E, Blank Lars M, Ibáñez Alfredo J, Sinues Pablo M-L
Department of Chemistry and Applied Biosciences, ETH Zurich, 8093, Zurich, Switzerland.
SEADM S.L., C\ José Lázaro Galdiano, 1, Madrid, 28036, Spain.
Sci Rep. 2017 Oct 27;7(1):14236. doi: 10.1038/s41598-017-14554-y.
While yeast is one of the most studied organisms, its intricate biology remains to be fully mapped and understood. This is especially the case when it comes to capture rapid, in vivo fluctuations of metabolite levels. Secondary electrospray ionization-high resolution mass spectrometry SESI-HRMS is introduced here as a sensitive and noninvasive analytical technique for online monitoring of microbial metabolic activity. The power of this technique is exemplarily shown for baker's yeast fermentation, for which the time-resolved abundance of about 300 metabolites is demonstrated. The results suggest that a large number of metabolites produced by yeast from glucose neither are reported in the literature nor are their biochemical origins deciphered. With the technique demonstrated here, researchers interested in distant disciplines such as yeast physiology and food quality will gain new insights into the biochemical capability of this simple eukaryote.
尽管酵母是研究最为深入的生物之一，但其复杂的生物学特性仍有待全面解析和深入理解。在捕捉代谢物水平快速的体内波动方面，情况尤其如此。本文引入了二次电喷雾电离-高分辨率质谱法（SESI-HRMS），作为一种用于在线监测微生物代谢活性的灵敏且非侵入性的分析技术。该技术的强大之处在面包酵母发酵中得到了例证，展示了约300种代谢物的时间分辨丰度。结果表明，酵母利用葡萄糖产生的大量代谢物在文献中既未被报道，其生化来源也未得到阐明。通过本文所展示的技术，对酵母生理学和食品质量等不同学科感兴趣的研究人员将对这种简单真核生物的生化能力获得新的认识。
