Shpichka Anastasia, Koroleva Anastasia, Kuznetsova Daria, Dmitriev Ruslan I, Timashev Peter
Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, Moscow, Russia.
Laser Zentrum Hannover e.V., Hannover, Germany.
Adv Exp Med Biol. 2017;1035:71-81. doi: 10.1007/978-3-319-67358-5_5.
Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a framework of their typical preparation and evaluation for tissue engineering. This chapter describes the fabrication of 3D scaffolds using two-photon polymerization, decellularization and cell encapsulation methods and easy-to-use protocols allowing assessing the cell morphology, cytotoxicity and viability in these scaffolds.
基于聚合物、陶瓷和混合材料的三维（3D）支架或基质结构对于成功的组织工程至关重要。虽然利用基于细胞的支架和基质结构的方法数量在不断增加，但为组织工程提供其典型制备和评估的框架至关重要。本章介绍了使用双光子聚合、去细胞化和细胞封装方法制造3D支架，以及便于评估这些支架中细胞形态、细胞毒性和活力的实用方案。
