使用多细胞球体模型和光片显微镜对细胞侵袭进行实时成像。

Live Imaging of Cell Invasion Using a Multicellular Spheroid Model and Light-Sheet Microscopy.

作者信息

Marcello Marco, Richards Rosalie, Mason David, Sée Violaine

机构信息

Department of Biochemistry and Centre for Cell Imaging, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.

出版信息

Adv Exp Med Biol. 2017;1035:155-161. doi: 10.1007/978-3-319-67358-5_11.

DOI:10.1007/978-3-319-67358-5_11

PMID:29080137

Abstract

Three-dimensional cellular assays are becoming increasingly popular as a fundamental tool to bridge the gap between tissue culture systems and in vivo tissue. In particular, spheroids are recognised today as a necessary intermediate model between testing in monolayer cultures and testing in animals. This chapter describes a straightforward protocol, from sample preparation to image acquisition and initial post-processing, based on one of most widely used commercial light-sheet fluorescence microscopy platform, the Zeiss Lightsheet Z.1.

摘要

三维细胞分析作为一种连接组织培养系统与体内组织之间差距的基础工具，正变得越来越受欢迎。特别是，如今球体被认为是单层培养测试和动物测试之间必要的中间模型。本章介绍了一种简单的方案，从样品制备到图像采集和初步后处理，该方案基于最广泛使用的商业光片荧光显微镜平台之一——蔡司光片Z.1。