Takahashi Taku, Takano Ayaka, Kakehi Jun-Ichi
Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University, Tsushimanaka 3-1-1, kita-ku, 700-8530, Okayama, Japan.
Methods Mol Biol. 2018;1694:69-73. doi: 10.1007/978-1-4939-7398-9_6.
Thermospermine, a structural isomer of spermine, is widely spread in the plant kingdom and has recently been shown to play a key role in the repression of xylem differentiation in vascular plants. However, a standard high-performance liquid chromatography (HPLC) protocol for detecting polyamines as their dansyl derivative cannot distinguish themospermine from spermine. These isomers become separated from each other after benzoylation. In this chapter, we describe a simple protocol for extraction, benzoylation, and HPLC detection of thermospermine and spermine with other polyamines from plant material.
热精胺是精胺的结构异构体，广泛分布于植物界，最近研究表明其在维管植物木质部分化的抑制过程中起关键作用。然而，用于检测作为丹磺酰衍生物的多胺的标准高效液相色谱（HPLC）方法无法区分热精胺和精胺。这些异构体在苯甲酰化后彼此分离。在本章中，我们描述了一种从植物材料中提取、苯甲酰化以及用HPLC检测热精胺、精胺和其他多胺的简单方法。
