Torras-Claveria Laura, Bastida Jaume, Viladomat Francesc, Tiburcio Antonio F
Department of Biology, Healthcare and Environment, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII 27-31, 08028, Barcelona, Spain.
Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Avda. Joan XXIII 27-31, 08028, Barcelona, Spain.
Methods Mol Biol. 2018;1694:95-104. doi: 10.1007/978-1-4939-7398-9_9.
Polyamines conjugated with hydroxycinnamic acids are phenolic compounds, which are widespread in the plant kingdom playing important roles in development and defence responses. This chapter describes the methodology employed to analyze these phenolamides in plant material by liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-MS-MS). These compounds are not always in sufficient concentration in plant tissues for analysis by more conventional methods such as UV detection of HPLC. Owing to their particular molecular structure, they cannot be analyzed as free polyamines. Thus, described herein is an extraction method for hydroxycinnamic acid amides in plant tissues such as leaves, and their analysis by LC-MS-MS, including identification and quantification protocols.
与羟基肉桂酸结合的多胺是酚类化合物，广泛存在于植物界，在植物发育和防御反应中发挥着重要作用。本章介绍了通过液相色谱-电喷雾电离串联质谱法（LC-MS-MS）分析植物材料中这些酚酰胺的方法。这些化合物在植物组织中的浓度往往不足以通过诸如高效液相色谱法的紫外检测等更传统的方法进行分析。由于其特殊的分子结构，它们不能作为游离多胺进行分析。因此，本文介绍了一种从叶片等植物组织中提取羟基肉桂酸酰胺的方法，以及通过LC-MS-MS对其进行分析的方法，包括鉴定和定量方案。
