Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Angew Chem Int Ed Engl. 2018 Apr 9;57(16):4329-4337. doi: 10.1002/anie.201709201. Epub 2018 Feb 28.
The discovery of the CRISPR-Cas9 endonuclease has enabled facile genome editing in living cells and organisms. Catalytically inactive Cas9 (dCas9) retains the ability to bind DNA in an RNA-guided fashion, and has additionally been explored as a tool for transcriptional modulation, epigenetic editing, and genome imaging. This Review highlights recent progress and challenges in the development of dCas9 for imaging genomic loci. The emergence and maturation of this technology offers the potential to answer mechanistic questions about chromosome dynamics and three-dimensional genome organization in vivo.
CRISPR-Cas9 内切酶的发现使在活细胞和生物体中进行基因组编辑变得更加容易。无酶活性的 Cas9（dCas9）保留了以 RNA 引导的方式结合 DNA 的能力，并且还被探索作为转录调节、表观遗传编辑和基因组成像的工具。本文综述了 dCas9 用于基因组定位成像的最新进展和挑战。该技术的出现和成熟为回答关于体内染色体动力学和三维基因组组织的机制问题提供了潜力。
