用于改进单链和双链CRISPR引导RNA设计的GuideScan软件。
GuideScan software for improved single and paired CRISPR guide RNA design.
作者信息
Perez Alexendar R, Pritykin Yuri, Vidigal Joana A, Chhangawala Sagar, Zamparo Lee, Leslie Christina S, Ventura Andrea
机构信息
Computational Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
出版信息
Nat Biotechnol. 2017 Apr;35(4):347-349. doi: 10.1038/nbt.3804. Epub 2017 Mar 6.
We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.
我们展示了用于设计CRISPR向导RNA文库的GuideScan软件，该文库可用于编辑编码和非编码基因组区域。GuideScan为全基因组单向导RNA和双向导RNA筛选生成高密度的向导RNA（gRNA）集。我们还表明，GuideScan的前缀树数据结构能够设计出比现有工具设计的gRNA更具特异性的gRNA。
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