Manivannan Bhavani, Kuppusamy Thayalan, Venkatesan Srinivasan, Perumal Venkatachalam
Department of Human Genetics, College of Biomedical Sciences, Technology and Research, Sri Ramachandra University, Porur, Chennai 600116, Tamil Nadu, India.
Dr. Kamakshi Memorial Hospital Pvt. Ltd., Pallikaranai, Chennai 600100, Tamil Nadu, India.
Appl Radiat Isot. 2018 Jan;131:1-7. doi: 10.1016/j.apradiso.2017.10.031. Epub 2017 Oct 16.
The γ-H2AX assay was investigated as an alternative to the time-consuming dicentric chromosome assay (DCA). Radiation doses to 25 radiotherapy patients were estimated in parallel by DCA and the γ-H2AX assay. The γ-H2AX assay yielded doses in line with the calculated equivalent whole body doses in 92% of the patients, whereas the success rate of DCA was only 76%. The result shows that the γ-H2AX assay can be effectively used as a rapid and more precise alternative to DCA.
对γ-H2AX检测法进行了研究，以替代耗时的双着丝粒染色体检测法（DCA）。通过DCA和γ-H2AX检测法并行估算了25名放疗患者的辐射剂量。γ-H2AX检测法得出的剂量与92%患者计算出的全身等效剂量一致，而DCA的成功率仅为76%。结果表明，γ-H2AX检测法可有效地作为DCA的一种快速且更精确的替代方法。
