Davis B J, Jang T
Department of Cell Biology and Anatomy, University of Alabama, Birmingham 35294.
Neuroscience. 1988 Dec;27(3):949-64. doi: 10.1016/0306-4522(88)90198-4.
A quantitative electron-microscopic analysis has been conducted on the neurons within the gustatory zone of the nucleus of the solitary tract of the hamster. The most common group of neurons within the gustatory zone contains both large (X1) and small (X3) members that possess deeply invaginated nuclear profiles. These neurons have somal areas that average 113 micron2 (range 34-281 micron2) and a value of somal area/nuclear area that averages 2.2. Other large and small neurons that have non-invaginated nuclear profiles are also observed. The larger (X2) neurons average 151 micron2 (range 49-487 micron2) and have much cytoplasm and associated membranous organelles that is reflected in a mean value of somal area/nuclear area of 2.6. Members of the X2 group are the largest neurons in the gustatory zone. The smaller (X4) group contains the smallest neurons in the gustatory zone of the nucleus of the solitary tract, averages 50 micron2 (range 16-103 micron2), shows almost no perinuclear cytoplasm and has a mean value of somal area/nuclear area of only 1.5. These findings are consistent with and expand upon the results of similar studies at the light-microscopic level. This grouping has been used to explore the association of tyrosine hydroxylase-like and dopamine beta-hydroxylase-like immunoreactivities with specific populations of neurons that are known to be distributed across the various levels of the gustatory zone. At the light-microscopic level, numerous well-defined and intensely labelled tyrosine hydroxylase-like immunoreactive somata of various morphologies and sizes are observed. Quantification at the electron-microscopic level indicates that 10-15% of the neurons encountered in the dorsal and intermediate levels of the gustatory zone are immunoreactive. The ventral level of the gustatory zone contains few immunoreactive neurons. Tyrosine hydroxylase-like immunoreactive neurons possess either non-invaginated or invaginated nuclear profiles and their somal areas average 106 and 142 micron2, respectively. On the bases of size and ultrastructural features, these immunoreactive somata are assigned to the two groups (X1 and X2) of large neurons within the gustatory portion of the nucleus of the solitary tract. In general, small neurons are not immunoreactive. The distribution of dopamine beta-hydroxylase-like immunoreactivity has also been examined in adjacent sections in order to reveal the presence of any putative noradrenergic neurons in the gustatory zone.(ABSTRACT TRUNCATED AT 400 WORDS)
对仓鼠孤束核味觉区内的神经元进行了定量电子显微镜分析。味觉区内最常见的神经元群体包含大（X1）、小（X3）两种类型，其核轮廓深陷。这些神经元的胞体面积平均为113平方微米（范围34 - 281平方微米），胞体面积与核面积之比平均为2.2。还观察到其他具有非深陷核轮廓的大、小神经元。较大的（X2）神经元平均为151平方微米（范围49 - 487平方微米），有大量细胞质和相关膜性细胞器，这反映在胞体面积与核面积的平均值为2.6。X2组的神经元是味觉区最大的神经元。较小的（X4）组包含孤束核味觉区最小的神经元，平均为50平方微米（范围16 - 103平方微米），几乎没有核周细胞质，胞体面积与核面积的平均值仅为1.5。这些发现与光镜水平的类似研究结果一致且有所扩展。这种分组已被用于探索酪氨酸羟化酶样和多巴胺β-羟化酶样免疫反应性与已知分布在味觉区不同水平的特定神经元群体的关联。在光镜水平，观察到许多形态和大小各异、定义明确且标记强烈的酪氨酸羟化酶样免疫反应性胞体。电子显微镜水平的定量分析表明，在味觉区背侧和中间水平遇到的神经元中有10% - 15%具有免疫反应性。味觉区腹侧水平含免疫反应性神经元较少。酪氨酸羟化酶样免疫反应性神经元具有非深陷或深陷的核轮廓，其胞体面积分别平均为106和142平方微米。基于大小和超微结构特征，这些免疫反应性胞体被归入孤束核味觉部分内的大神经元的两组（X1和X2）。一般来说，小神经元无免疫反应性。还在相邻切片中检查了多巴胺β-羟化酶样免疫反应性的分布，以揭示味觉区中是否存在任何假定的去甲肾上腺素能神经元。（摘要截于400字）
