Poverennaya E V, Kiseleva O I, Ponomarenko E A, Naryzhny S N, Zgoda V G, Lisitsa A V
Institute of Biomedical Chemistry, Moscow, Russia.
Biomed Khim. 2017 Oct;63(5):373-378. doi: 10.18097/PBMC20176305373.
Current proteomic studies are generally focused on the most abundant proteoforms encoded by canonical nucleic sequences. Transcriptomic and proteomic data, accumulated in a variety of postgenome sources and coupled with state-of-art analytical technologies, allow to start the identification of aberrant (non-canonical) proteoforms. The main sources of aberrant proteoforms are alternative splicing, single nucleotide polymorphism, and post-translational modifications. The aim of this work was to estimate the heterogeneity of HepG2 proteome. We suggested multiomics approach, which combines transcriptomic (RNAseq) and proteomic (2DE-MS/MS) methods, as a promising strategy to explore the proteome.
当前的蛋白质组学研究通常聚焦于由标准核酸序列编码的最丰富的蛋白质异构体。转录组学和蛋白质组学数据积累于各种后基因组来源，并与先进的分析技术相结合，使得开始识别异常（非标准）蛋白质异构体成为可能。异常蛋白质异构体的主要来源是可变剪接、单核苷酸多态性和翻译后修饰。这项工作的目的是评估HepG2蛋白质组的异质性。我们提出了一种多组学方法，该方法结合了转录组学（RNA测序）和蛋白质组学（二维电泳-串联质谱）方法，作为探索蛋白质组的一种有前景的策略。
