Institute of Biomedical Chemistry, Moscow 119121, Russia.
Int J Mol Sci. 2020 May 27;21(11):3813. doi: 10.3390/ijms21113813.
One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to catalog and annotate a myriad of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve a detailed and personalized understanding into proteomes, we suggest using a customized RNA-seq library of potential proteoforms, which includes aberrant variants specific to certain biological samples. Two-dimensional electrophoresis coupled with high-performance liquid chromatography allowed us to downgrade the difficulty of biological mixing following shotgun mass spectrometry. To benchmark the proposed pipeline, we examined heterogeneity of the HepG2 hepatoblastoma cell line proteome. Data are available via ProteomeXchange with identifier PXD018450.
染色体为中心的人类蛋白质组计划（C-HPP）的主要目标之一是对大约 20000 个基因产生的无数异质蛋白进行编目和注释。为了深入了解蛋白质组，我们建议使用定制的潜在蛋白的 RNA-seq 文库，其中包括特定于某些生物样本的异常变体。二维电泳与高效液相色谱相结合，使得我们能够在进行鸟枪法质谱分析之前降低生物混合物的难度。为了验证所提出的管道，我们检查了 HepG2 肝癌细胞系蛋白质组的异质性。数据可通过 ProteomeXchange 获取，标识符为 PXD018450。
