德国石勒苏益格-荷尔斯泰因州猪肠道病毒G的高度遗传多样性。
High genetic diversity of porcine enterovirus G in Schleswig-Holstein, Germany.
作者信息
Bunke Jennifer, Receveur Kerstin, Oeser Ann Christin, Fickenscher Helmut, Zell Roland, Krumbholz Andi
机构信息
Institut für Infektionsmedizin, Christian-Albrechts-Universität zu Kiel und Universitätsklinikum Schleswig-Holstein, Brunswiker Straße 4, 24105, Kiel, Germany.
Sektion für Experimentelle Virologie, Institut für Medizinische Mikrobiologie, Friedrich Schiller Universität Jena und Universitätsklinikum Jena, Hans-Knöll-Straße 2, 07743, Jena, Germany.
出版信息
Arch Virol. 2018 Feb;163(2):489-493. doi: 10.1007/s00705-017-3612-x. Epub 2017 Oct 28.
Between 2012 and 2015, 495 pooled snout swabs from fattening pigs raised in Schleswig-Holstein, Germany, were screened for the presence of enterovirus G (EV-G) RNA. Nucleic acids were tested in diverse reverse transcription polymerase chain reaction assays applying published oligonucleotide primers specific for the viral protein (VP) 1 and 2/4 encoding regions as well as for 3D polymerase. Phylogenetic analyses of VP1 revealed the presence of 12 EV-G types, three of which had highly divergent sequences suggesting putative new types. Co-circulation of EV-G types was observed in several pigsties. Thus, genetic diversity of EV-G was demonstrated in this small geographic area.
2012年至2015年间，对德国石勒苏益格－荷尔斯泰因州饲养的育肥猪的495份合并鼻拭子进行了肠道病毒G（EV-G）RNA检测。采用针对病毒蛋白（VP）1和2/4编码区以及3D聚合酶的已发表寡核苷酸引物，在多种逆转录聚合酶链反应检测中对核酸进行了检测。VP1的系统发育分析显示存在12种EV-G型，其中三种具有高度不同的序列，提示可能为新型。在几个猪舍中观察到了EV-G型的共同循环。因此，在这个小地理区域内证明了EV-G的遗传多样性。
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