Zhu Pei, Li Zhan-Hong, Li Zhuo-Ran, Zhang Zhen-Xing, Song Jian-Ling
Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, China.
Key Laboratory of Transboundary Animal Diseases Prevention and Control (Co-Construction by Ministry and Province), Yunnan Animal Science and Veterinary Institute, Kunming, China.
Front Vet Sci. 2024 Jul 24;11:1431180. doi: 10.3389/fvets.2024.1431180. eCollection 2024.
Enterovirus G (EV-G) belongs to the Picornaviridae family and infects porcine populations worldwide. A total of 20 EV-G genotypes (EV-G1 to EV-G20) have been identified. In this study, we isolated and characterized an EV-G strain, named EV-G/YN29/2022, from the feces of diarrheic pigs. This was the first EV-G6 strain isolated in China. Comparison of the whole genome nucleotide and corresponding amino acid sequences showed that the isolate was more closely related to those of the EV-G6 genotype than other genotypes, with the complete genome sequence similarity ranging from 83.7% (Iba46442) to 84.4% (PEV-B-KOR), and corresponding amino acid homology ranged from 96% (Iba46442) to 96.8% (PEV-B-KOR). Similarly, the VP1 gene and corresponding amino acid sequences of EV-G/YN29/2022 were highly similar to those of the EV-G6 genotype (>82.9% and >94.3%, respectively). Thus, the isolated strain was classified as EV-G6 genotype. This was the first EV-G6 strain isolated in China. Pathogenicity analyses revealed that EV-G/YN29/2022 infection caused mild diarrhea, typical skin lesions, and weight reduction. The strain was mainly distributed to the intestinal tissue but was also found in the brain, mesenteric lymph nodes, spleen, and liver. Our results can be used as a reference to further elucidate the epidemiology, evolution, and pathogenicity of EV-G.
肠道病毒G（EV-G）属于小RNA病毒科，可感染全球范围内的猪群。目前已鉴定出总共20种EV-G基因型（EV-G1至EV-G20）。在本研究中，我们从腹泻猪的粪便中分离并鉴定了一株EV-G毒株，命名为EV-G/YN29/2022。这是中国分离出的第一株EV-G6毒株。全基因组核苷酸和相应氨基酸序列的比较表明，该分离株与EV-G6基因型的毒株比其他基因型更为密切相关，完整基因组序列相似性范围为83.7%（Iba46442）至84.4%（PEV-B-KOR），相应氨基酸同源性范围为96%（Iba46442）至96.8%（PEV-B-KOR）。同样，EV-G/YN29/2022的VP1基因和相应氨基酸序列与EV-G6基因型的高度相似（分别>82.9%和>94.3%）。因此，该分离株被归类为EV-G6基因型。这是中国分离出的第一株EV-G6毒株。致病性分析显示，EV-G/YN29/2022感染导致轻度腹泻、典型皮肤病变和体重减轻。该毒株主要分布于肠道组织，但也在脑、肠系膜淋巴结、脾脏和肝脏中被发现。我们的结果可为进一步阐明EV-G的流行病学、进化和致病性提供参考。
