McLafferty M A, Harcus D R, Hewlett E L
Department of Medicine, University of Virginia School of Medicine, Charlottesville 22908.
J Gen Microbiol. 1988 Aug;134(8):2297-306. doi: 10.1099/00221287-134-8-2297.
A repeating element of DNA has been isolated and sequenced from the genome of Bordetella pertussis. Restriction map analysis of this element shows single internal ClaI, SphI, BstEII and SalI sites. Over 40 DNA fragments are seen in ClaI digests of B. pertussis genomic DNA to which the repetitive DNA sequence hybridizes. Sequence analysis of the repeat reveals that it has properties consistent with bacterial insertion sequence (IS) elements. These properties include its length of 1053 bp, multiple copy number and presence of 28 bp of near-perfect inverted repeats at its termini. Unlike most IS elements, the presence of this element in the B. pertussis genome is not associated with a short duplication in the target DNA sequence. This repeating element is not found in the genomes of B. parapertussis or B. bronchiseptica. Analysis of a DNA fragment adjacent to one copy of the repetitive DNA sequence has identified a different repeating element which is found in nine copies in B. parapertussis and four copies in B. pertussis, suggesting that there may be other repeating DNA elements in the different Bordetella species. Computer analysis of the B. pertussis repetitive DNA element has revealed no significant nucleotide homology between it and any other bacterial transposable elements, suggesting that this repetitive sequence is specific for B. pertussis.
一种重复的DNA元件已从百日咳博德特氏菌的基因组中分离并测序。对该元件的限制性图谱分析显示有单个内部ClaI、SphI、BstEII和SalI位点。在百日咳博德特氏菌基因组DNA的ClaI消化产物中可见40多个与重复DNA序列杂交的DNA片段。对该重复序列的序列分析表明，它具有与细菌插入序列（IS）元件一致的特性。这些特性包括其长度为1053 bp、多拷贝数以及在其末端存在28 bp近乎完美的反向重复序列。与大多数IS元件不同，该元件在百日咳博德特氏菌基因组中的存在与靶DNA序列中的短重复无关。在副百日咳博德特氏菌或支气管败血博德特氏菌的基因组中未发现这种重复元件。对与一个重复DNA序列拷贝相邻的DNA片段进行分析，鉴定出一种不同的重复元件，该元件在副百日咳博德特氏菌中有九个拷贝，在百日咳博德特氏菌中有四个拷贝，这表明在不同的博德特氏菌物种中可能存在其他重复DNA元件。对百日咳博德特氏菌重复DNA元件的计算机分析显示，它与任何其他细菌转座元件之间没有显著的核苷酸同源性，这表明该重复序列是百日咳博德特氏菌特有的。
