从百日咳博德特氏菌基因组中分离出具有插入序列元件特征的重复序列。

Isolation of a repeated sequence from the genome of Bordetella parapertussis with characteristics of an insertion sequence element.

作者信息

van der Zee A, Agterberg C, van Agterveld M, Peeters M, Mooi F

机构信息

Laboratory of Medical Microbiology, St. Elisabeth Hospital, Tillburg, The Netherlands.

出版信息

Zentralbl Bakteriol. 1993 Apr;278(2-3):451-5. doi: 10.1016/s0934-8840(11)80862-5.

DOI:10.1016/s0934-8840(11)80862-5

PMID:8394166

Abstract

A repeated DNA sequence in the genome of Bordetella parapertussis was identified by means of heteroduplex formation and S1 nuclease treatment. Cloning and sequencing of the repeated sequence, revealed that it comprised 1306 basepairs, contained inverted repeats at its termini, and two large open reading frames. This insertion sequence element was designated IS1001, and was found in about 20 copies in 10 B. parapertussis strains analyzed. Identical hybridization patterns were observed in B. parapertussis when IS1001 was used as a probe. B. pertussis strains did not hybridize to IS1001.

摘要

通过异源双链体形成和S1核酸酶处理，在副百日咳博德特氏菌基因组中鉴定出一个重复DNA序列。对该重复序列进行克隆和测序后发现，它由1306个碱基对组成，在其末端含有反向重复序列以及两个大的开放阅读框。这个插入序列元件被命名为IS1001，在所分析的10株副百日咳博德特氏菌菌株中约有20个拷贝。当使用IS1001作为探针时，在副百日咳博德特氏菌中观察到相同的杂交模式。百日咳博德特氏菌菌株与IS1001不杂交。