Ahmadi Amin, Borji Hassan, Naghibi Abolghasem, Nasiri Mohammad Reza, Sharifiyazdi Hassan
Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, PO Box 9177948974, Mashhad, Iran (Ahmadi, Borji, Naghibi); Department of Animal Sciences, School of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran (Nasiri); Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran (Sharifiyazdi).
Can J Vet Res. 2017 Oct;81(4):280-284.
Dactylogyrids are a group of monogenean parasites that have a high species intensity on the gills of cyprinid fish. In this study, 89 common carp and 25 grass carp were collected from Mashhad, in northeastern Iran. A total of 31 specimens, 20 and 11 specimens from the gills of common carp and grass carp, respectively, were collected and studied by morphologic analysis and molecular analysis based on 28S rDNA. Four lineages were revealed: and a new species. Phylogenetic analysis showed that 2 species, namely and were identical to those previously reported. In addition, nucleotide sequencing showed the greatest homology (93.01%) of to be with a species registered as in the Basic Local Alignment Search Tool database. The new species formed a distinct clade of its own in the phylogram. From the morphologic, molecular, and phylogenetic evidence, we propose that this isolate is a single new species within the genus Further phylogenetic analysis, however, including the incorporation of additional molecular targets, is required to infer relationships among species in the genus.
指环虫是一类单殖吸虫寄生虫，在鲤科鱼类的鳃上具有较高的物种密度。在本研究中，从伊朗东北部的马什哈德采集了89条鲤鱼和25条草鱼。总共收集了31个标本，分别从鲤鱼和草鱼的鳃上采集了20个和11个标本，并基于28S rDNA进行形态学分析和分子分析。揭示了四个谱系：以及一个新物种。系统发育分析表明，有两个物种，即和与先前报道的物种相同。此外，核苷酸测序显示，与基本局部比对搜索工具数据库中注册为的一个物种具有最高的同源性（93.01%）。新的物种在系统发育树中形成了一个独特的分支。从形态学、分子学和系统发育学证据来看，我们认为这个分离株是属内的一个单一新物种。然而，需要进一步的系统发育分析，包括纳入更多的分子靶点，以推断属内物种之间的关系。
