Wang Jing, Zhi Chan-Ping, Chen Xiao-Jie, Guo Ze-Wen, Liu Wu-Ling, Luo Juan, Huang Xin-Yi, Zeng Li, Huang Jia-Wei, Xia Ying-Bi, Yi Meng-Ying, Huang Teng, Zeng Zhen-Ling, Liu Jian-Hua
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2017 Oct 13;8:1982. doi: 10.3389/fmicb.2017.01982. eCollection 2017.
The purpose of this study was to investigate the prevalence and genetic elements of among isolates from animals, retail meat, and humans (patients with infection or colonization) in Guangzhou, China. A total of 1,354 isolates were screened for by PCR. Fifty -positive isolates were further characterized by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), S1-PFGE, genetic environment analysis, plasmid replicon typing, and plasmid sequencing. was detected in 172 (33.79%), 60 (17.34%), and 90 (18.07%) isolates from animal, food, and human, respectively. High clonal diversity was observed among -positive isolates. In 21 -containing transformants, was flanked by two IS elements in the same orientation, formed a composite transposon Tn in 19 transformants, and was located on plasmids (33.3~500 kb) belonging to IncN1-F33:A-:B- ( = 3), IncHI2/ST3 ( = 3), F-:A18:B- ( = 2), F-:A-:B54 ( = 2), or others. Additionally, was co-located with multiple resistance genes on the same plasmid, such as ' and/or , which were identified in two F-:A18:B- plasmids from pigs, and , , and , which were detected in two N1-F33:A-:B- plasmids from patients. The two IncHI2/ST3 -bearing plasmids, pHNLDF400 and pHNYJC8, which were isolated from human patient and chicken meat, respectively, contained a typical IncHI2-type backbone, and were similar to each other with 2-bp difference, and also showed 99% identity to the Typhimurium -carrying plasmids pHXY0908 (chicken) and pHK0653 (human patient). Horizontal transfer mediated by mobile elements may be the primary mechanism underlying spread in isolates obtained from various sources in Guangzhou, China. The transmission of identical -carrying IncHI2 plasmids between food products and humans might pose a serious threat to public health.
本研究旨在调查中国广州动物、零售肉类及人类（感染或定植患者）分离株中[具体细菌名称未给出]的流行情况及遗传元件。通过PCR对总共1354株[具体细菌名称未给出]分离株进行筛选。对50株阳性分离株进一步采用脉冲场凝胶电泳（PFGE）、多位点序列分型（MLST）、S1-PFGE、遗传环境分析、质粒复制子分型及质粒测序进行特征分析。在分别来自动物、食品和人类的[具体细菌名称未给出]分离株中，检测到[具体细菌名称未给出]的比例分别为172株（33.79%）、60株（17.34%）和90株（18.07%）。在阳性分离株中观察到高度的克隆多样性。在21株含有[具体细菌名称未给出]的转化体中，[具体细菌名称未给出]两侧有两个同向的IS元件，在19个转化体中形成复合转座子Tn，并位于属于IncN1-F33:A-:B-（n = 3）、IncHI2/ST3（n = 3）、F-:A18:B-（n = 2）、F-:A-:B54（n = 2）或其他类型的质粒（33.3~500 kb）上。此外，[具体细菌名称未给出]与同一质粒上的多个耐药基因共定位，如在来自猪的两个F-:A18:B-质粒中鉴定出的‘和/或’，以及在来自患者的两个N1-F33:A-:B-质粒中检测到的、、和。分别从人类患者和鸡肉中分离得到的两个携带IncHI2/ST3 [具体细菌名称未给出]的质粒pHNLDF400和pHNYJC8，含有典型的IncHI2型骨架，彼此相似，仅有2个碱基差异，并且与携带[具体细菌名称未给出]的鼠伤寒沙门氏菌质粒pHXY0908（鸡肉）和pHK0653（人类患者）也有99%的同一性。由移动元件介导的水平转移可能是中国广州从各种来源获得的[具体细菌名称未给出]分离株中[具体细菌名称未给出]传播的主要机制。携带相同[具体细菌名称未给出]的IncHI2质粒在食品和人类之间的传播可能对公共卫生构成严重威胁。
