人血浆生长抑素固相放射免疫分析法的研制。

Development of a solid phase RIA for human plasma somatostatin.

作者信息

Brocchi A, Tommasi M

机构信息

Dipartimento di Fisiopatologia Clinica, Unità di Medicina Nucleare, Firenze, Italy.

出版信息

Int J Rad Appl Instrum B. 1988;15(5):553-5. doi: 10.1016/s0969-8051(88)80014-3.

DOI:10.1016/s0969-8051(88)80014-3

PMID:2908203

Abstract

Somatostatin is widely distributed in tissues of biological fluids. In the present study we tested the validity of a new radioimmunoassay by a solid phase immunoextraction. Sensitivity was 1.8 pg/mL and the within-assay precision with two different pools of human plasma was 12.2% (CV) at a concentration of 19.2 +/- 2.34 pg/mL (mean +/- SD) and 11.9% at 8.53 +/- 1.02 pg/mL. The between assay precision was 14.2% (CV) at a concentration of 11.8 +/- 1.68 pg/mL. The accuracy was good as tested by the dilution test (slope: 0.956, Y-intercept 3.07 pg/mL, r = 0.997) and the recovery test (slope: 1.008, Y-intercept 2.22 pg/mL, r = 0.999). The method resulted in good correlation with a current extraction method (no. 65, y = 1.45 + 0.975 x, r = 0.862, P less than 0.001). Basal value of somatostatin in the plasma of 32 normal subjects was 11.5 +/- 5.80 pg/mL (mean +/- SD).

摘要

生长抑素广泛分布于生物体液组织中。在本研究中，我们通过固相免疫萃取法测试了一种新的放射免疫分析法的有效性。灵敏度为1.8 pg/mL，在两个不同的人血浆样本池中，当浓度为19.2±2.34 pg/mL（平均值±标准差）时，批内精密度为12.2%（变异系数），当浓度为8.53±1.02 pg/mL时，批内精密度为11.9%。在浓度为11.8±1.68 pg/mL时，批间精密度为14.2%（变异系数）。通过稀释试验（斜率：0.956，Y轴截距3.07 pg/mL，r = 0.997）和回收率试验（斜率：1.008，Y轴截距2.22 pg/mL，r = 0.999）测试，准确性良好。该方法与当前的萃取方法（编号65，y = 1.45 + 0.975 x，r = 0.862，P＜0.001）具有良好的相关性。32名正常受试者血浆中生长抑素的基础值为11.5±5.80 pg/mL（平均值±标准差）。