Lundqvist G, Gustavsson S, Elde R, Arimura A
Clin Chim Acta. 1980 Feb 28;101(2-3):183-91. doi: 10.1016/0009-8981(80)90243-0.
A solid phase radioimmunoassay for determination of immunoreactive somatostatin (IRS) in plasma is described. Plasma samples obtained from 30 apparently healthy persons and from 5 anaesthetized pigs were extracted with acetone-petroleum ether. The antibodies (R 141 and R 101) were conjugated to cyanogenbromide activated microcrystalline cellulose. Tyr1-somatostatin was iodinated according to the lactoperoxidase method. After extraction the recovery of somatostatin varied between 80 and 118%. The sensitivity of the assay was 5--10 pg/ml and the intra- and interassay variation ranged between 8 and 20%. The mean (+/- S.D.) value of IRS in systemic blood in man was 77 (+/- 19) pg/ml. Intravenous administration of 10 micrograms/kg synthetic somatostatin to anaesthetized pigs was followed by a 20-fold increase in plasma IRS. The hypersomatostatinemia rapidly vanished with a half-life of 3.5 min. The level of IRS in cerebrospinal fluid was unchanged by intravenous somatostatin at this dose.
本文描述了一种用于测定血浆中免疫反应性生长抑素（IRS）的固相放射免疫分析方法。从30名看似健康的人和5只麻醉猪身上采集的血浆样本用丙酮 - 石油醚进行提取。抗体（R 141和R 101）与溴化氰活化的微晶纤维素偶联。根据乳过氧化物酶法对酪氨酸 - 1 - 生长抑素进行碘化。提取后，生长抑素的回收率在80%至118%之间。该分析方法的灵敏度为5 - 10 pg/ml，批内和批间变异范围在8%至20%之间。人体全身血液中IRS的平均（±标准差）值为77（±19）pg/ml。给麻醉猪静脉注射10μg/kg合成生长抑素后，血浆IRS增加了20倍。高生长抑素血症迅速消失半衰期为3.5分钟。在此剂量下，静脉注射生长抑素后脑脊液中IRS水平未发生变化。
