Laboratory of Nutritional Pathophysiology, National Institute of Digestive Diseases, IRCCS "Saverio de Bellis", Castellana Grotte, Bari, Italy.
Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, Bari, Italy.
Biomed Res Int. 2017;2017:3178263. doi: 10.1155/2017/3178263. Epub 2017 Sep 7.
To investigate whether pathophysiological differences exist among healthy controls (HC) and patients with slow and normal transit constipation (STC and NTC), we evaluated (1) gastrointestinal (GI) symptoms using validated questionnaires; (2) circulating concentrations of neurotensin, motilin, corticotrophin-releasing factor (CRF), and somatostatin; and (3) possible differences in frequency distribution of the neurotensin rs1800832 A/G and Neurotensin Receptor 1 rs6090453 C/G SNPs.
Fifty-one patients with severe functional constipation and 20 HC completed the study. Symptoms were evaluated by GSRS and Constipaq scoring system. Plasma concentrations of GI peptides were evaluated by ELISA on fasting and six sequential blood samples after a standard meal. Genotyping was performed by PCR and endonuclease digestion.
Symptom profiles largely overlapped between NTC and STC patients. As for peptide profiles, neurotensin showed lower concentrations at 60 and 90 min in STC versus HC, and motilin showed throughout the curve 85% and 82% lower levels in STC than HC and NTC, respectively. Finally, neurotensin polymorphism resulted in being associated with the peptide levels.
Symptom profile is not a reliable tool to discriminate STC, whilst the GI peptide profiles might help in identifying it.
为了探究健康对照者（HC）和慢传输型便秘（STC）及正常传输型便秘（NTC）患者之间是否存在生理学差异，我们评估了（1）使用经过验证的问卷评估胃肠道（GI）症状；（2）循环中的神经降压素、胃动素、促肾上腺皮质释放因子（CRF）和生长抑素浓度；（3）神经降压素 rs1800832 A/G 和神经降压素受体 1 rs6090453 C/G SNP 的频率分布的可能差异。
51 名严重功能性便秘患者和 20 名 HC 完成了这项研究。通过 GSRS 和 Constipaq 评分系统评估症状。通过 ELISA 在空腹和标准餐后 6 个连续的血样中评估 GI 肽的血浆浓度。通过 PCR 和内切酶消化进行基因分型。
NTC 和 STC 患者的症状谱大部分重叠。就肽谱而言，神经降压素在 STC 患者中 60 和 90 分钟时的浓度低于 HC，而胃动素在 STC 患者中的浓度分别比 HC 和 NTC 低 85%和 82%。最后，神经降压素多态性与肽水平相关。
症状谱不能作为区分 STC 的可靠工具，而 GI 肽谱可能有助于识别它。
