Institute of Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovakia.
Institute of Process Engineering, Faculty of Mechanical Engineering, Slovak University of Technology in Bratislava, Bratislava, Slovakia.
J Sep Sci. 2018 Jan;41(2):449-458. doi: 10.1002/jssc.201700715. Epub 2017 Nov 27.
A simple method for the simultaneous derivatization of carbohydrates, polyols, amines and amino acids using hexamethyldisilazane and N,O-bis(trimethylsilyl)trifluoroacetamide was developed. This method allows the direct derivatization of urine samples without sample pretreatment before derivatization. The method was successfully used for analysis of the selected metabolites in urine samples of healthy individuals and neonates suffering from galactosemia. The limits of detection by positive chemical ionization gas chromatography with tandem mass spectrometry analysis were in the range of 1.0 mgL for mannitol to 4.7 mg/L for glucose.
开发了一种使用六甲基二硅氮烷和 N,O-双(三甲基硅基)三氟乙酰胺同时衍生碳水化合物、多元醇、胺和氨基酸的简单方法。该方法允许在衍生化前无需对尿液样本进行预处理，即可直接对尿液样本进行衍生化。该方法成功用于分析健康个体和患有半乳糖血症的新生儿尿液样本中的选定代谢物。正化学电离气相色谱-串联质谱分析的检测限范围为甘露醇 1.0mg/L 至葡萄糖 4.7mg/L。
