Wu Wen, Liang Kai-Lun, Chen Bo, Su Jie, Chen Su-Hong, Lyu Gui-Yuan
Zhejiang Chinese Medical University, Hangzhou 310053, China.
Zhejiang University of Technology, Hangzhou 310014, China.
Zhongguo Zhong Yao Za Zhi. 2017 May;42(9):1757-1761. doi: 10.19540/j.cnki.cjcmm.2017.0069.
To study the anti-obesity effect of Mori Folium extract on diet-induced obesity(DIO) and to explore the preliminary mechanism in rats. DIO rat models were established by high glucose and high fat diet for 8 weeks. Then high(10 mg•kg⁻¹) and low(5 mg•kg⁻¹) does Mori Folium extracts were given by intragastric administration for 13 weeks. After the last administration, their body weight, 24 h food intake, water intake, Lee's index, liver/body mass index, and fat/body mass index were determined. The levels of lipoprotein lipase(LPL), CCAAT/enhancer-binding protein alpha(C/EBPα) and peroxisome proliferator-activated receptor gamma(PPARγ) were measured by enzyme-linked immunosorbent assay(ELISA). Phosphorylated AMP-activated protein kinase alpha(p-AMPKα), C/EBPα and PPARγ expression levels in adipose tissues were detected by Western blot. The hematoxylin-eosin staining(HE) was used to observe the histopathological changes of adipose tissues. The results showed that both high dose and low dose Mori Folium extract can decrease body weight, Lee's index, renal fat/body mass ratio and testicle fat/body mass ratio, and the high dose group could decrease the total fat/body mass ratio. Both high dose and low dose groups had no significant effect on the food intake and water intake; however, they could decrease levels of LPL in fat, up-regulate p-AMPKα protein expression, down-regulate C/EBPα and PPARγ protein expression, and reduce fat cell volume. In conclusion, Mori Folium extract had a slimming effect on DIO rats, and its mechanism may be associated with up-regulating the expression of p-AMPKα, down-regulating the expression of PPARγ, C/EBPα and LPL, inhibiting the differentiation of preadipocytes into mature fat cells, and reducing the volume of fat cells.
研究桑树叶提取物对饮食诱导肥胖（DIO）大鼠的抗肥胖作用，并探讨其初步机制。通过高糖高脂饮食8周建立DIO大鼠模型。然后分别给予高剂量（10 mg•kg⁻¹）和低剂量（5 mg•kg⁻¹）的桑树叶提取物灌胃13周。末次给药后，测定大鼠体重、24小时食物摄入量、饮水量、李氏指数、肝体质量指数和脂肪体质量指数。采用酶联免疫吸附测定（ELISA）法检测脂蛋白脂肪酶（LPL）、CCAAT增强子结合蛋白α（C/EBPα）和过氧化物酶体增殖物激活受体γ（PPARγ）的水平。通过蛋白质免疫印迹法检测脂肪组织中磷酸化的AMP激活蛋白激酶α（p-AMPKα）、C/EBPα和PPARγ的表达水平。采用苏木精-伊红染色（HE）观察脂肪组织的组织病理学变化。结果显示，高剂量和低剂量桑树叶提取物均可降低体重、李氏指数、肾周脂肪体质量比和睾丸脂肪体质量比，高剂量组还可降低总脂肪体质量比。高剂量和低剂量组对食物摄入量和饮水量均无显著影响；但均可降低脂肪组织中LPL水平，上调p-AMPKα蛋白表达，下调C/EBPα和PPARγ蛋白表达，并减小脂肪细胞体积。综上所述，桑树叶提取物对DIO大鼠具有减肥作用，其机制可能与上调p-AMPKα表达、下调PPARγ、C/EBPα和LPL表达、抑制前脂肪细胞分化为成熟脂肪细胞以及减小脂肪细胞体积有关。
