Zhao Qingsong, Wang Lipei, Wu Xiongbin, Liu Tingting, Xu Jinmei, Li Ming, Li Bo, Guo Min, Cheng Zhifeng
Department of Endocrinology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
Department of Anesthesiology, No.211 Hospital of PLA, Harbin, China.
Int J Rheum Dis. 2018 Jan;21(1):330-337. doi: 10.1111/1756-185X.13181. Epub 2017 Oct 30.
This study aimed to explore the regulatory mechanism of the natural soda Shi Han Quan (SHQ) in the development of gout.
Human umbilical vein endothelial cells (HUVECs) were stimulated with monosodium urate (MSU) for 24 h to induce acute gouty inflammation in vitro. HUVECs were divided into four groups: ddH O group, ddH O + MSU group, 1/2 ddH O +1/2 SHQ + MSU group, and SHQ + MSU group. The effects of SHQ on cell viability, concentration and expression of intercellular adhesion molecule-1 (ICAM-1), and MSU-induced release of interleukin (IL)-1β and IL-6 were investigated. Additionally, cell viability and ICAM-1 concentration and expression were detected after HUVECs were incubated with the culture supernatant of THP-1 cells that had been treated with phorbol 12-myristate 13-acetate (PMA), MSU and SHQ for 24 h.
The viability of HUVECs was significantly decreased with increasing transfection concentrations of MSU, and MSU treatment resulted in a significant increase of the concentration and expression of ICAM-1. In addition, SHQ improved the MSU-induced decrease in cell viability and alleviated MSU-mediated increase in ICAM-1 levels. Moreover, SHQ decreased MSU-induced release of IL-1β and IL-6. After HUVECs were incubated with the culture supernatant of THP-1 cells that had been treated with PMA, MSU and SHQ for 24 h, SHQ also markedly alleviated the effects of MSU, such as by increasing cell viability and decreasing ICAM-1 levels.
Drinking natural soda water may have a significant role in preventing gouty inflammation.
本研究旨在探讨天然苏打水石汗泉（SHQ）在痛风发展中的调节机制。
用尿酸钠（MSU）刺激人脐静脉内皮细胞（HUVECs）24小时，以在体外诱导急性痛风性炎症。将HUVECs分为四组：双蒸水组、双蒸水+MSU组、1/2双蒸水+1/2 SHQ + MSU组和SHQ + MSU组。研究SHQ对细胞活力、细胞间黏附分子-1（ICAM-1）的浓度和表达以及MSU诱导的白细胞介素（IL）-1β和IL-6释放的影响。此外，在用佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）、MSU和SHQ处理24小时的THP-1细胞的培养上清液孵育HUVECs后，检测细胞活力以及ICAM-1的浓度和表达。
随着MSU转染浓度的增加，HUVECs的活力显著降低，且MSU处理导致ICAM-1的浓度和表达显著增加。此外，SHQ改善了MSU诱导的细胞活力下降，并减轻了MSU介导的ICAM-1水平升高。而且，SHQ降低了MSU诱导的IL-1β和IL-6释放。在用PMA、MSU和SHQ处理24小时的THP-1细胞的培养上清液孵育HUVECs后，SHQ也显著减轻了MSU的作用，如增加细胞活力和降低ICAM-1水平。
饮用天然苏打水可能在预防痛风性炎症方面具有重要作用。
