Emokpae Mathias Abiodun, Mrakpor Beatrice Aghogho
Department of Medical Laboratory Science, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin 300001, Nigeria.
Med Sci (Basel). 2016 Nov 15;4(4):19. doi: 10.3390/medsci4040019.
Studies have shown that human immunodeficiency virus type 1 (HIV-1) disproportionally affects more females than males. Affected individuals are susceptible to infections due to depressed immunity, qualitative defects in phagocytic function and altered phagocytosis as well as lowered oxidative burst capacity. This study seeks to determine whether sex differences exist in serum activities of respiratory burst enzymes in HIV-1-infected female and male subjects. Serum myeloperoxidase, catalase and superoxide dismutase activities were assayed in 170 confirmed HIV-1 positive and 50 HIV-1 negative subjects using ELISA. Data were analyzed using Student's -test and values of less than 0.05 were considered significant. The measured enzyme activities were significantly higher ( < 0.001) in females than males in HIV-1 negative subjects while no sex differences were observed in HIV-1 positive subjects. The absence of sex differences in the activities of respiratory burst enzymes in HIV-1 infection may be due to immune activation as a result of active phagocytic leukocytes, immune reactivity and inflammation.
研究表明，1型人类免疫缺陷病毒（HIV-1）对女性的影响比对男性的影响更大。由于免疫力低下、吞噬功能的质量缺陷、吞噬作用改变以及氧化爆发能力降低，受影响的个体易受感染。本研究旨在确定HIV-1感染的女性和男性受试者的血清呼吸爆发酶活性是否存在性别差异。使用酶联免疫吸附测定法（ELISA）对170名确诊的HIV-1阳性受试者和50名HIV-1阴性受试者的血清髓过氧化物酶、过氧化氢酶和超氧化物歧化酶活性进行了测定。数据采用学生t检验进行分析，p值小于0.05被认为具有统计学意义。在HIV-1阴性受试者中，女性的测量酶活性显著高于男性（p<0.001），而在HIV-1阳性受试者中未观察到性别差异。HIV-1感染中呼吸爆发酶活性不存在性别差异可能是由于活跃的吞噬性白细胞、免疫反应性和炎症导致的免疫激活。
