Lim Juha, Kale Madhuvanti, Kim Dong-Hyeon, Kim Hong-Seok, Chon Jung-Whan, Seo Kun-Ho, Lee Hyeon Gyu, Yokoyama Wallace, Kim Hyunsook
Department of Food and Nutrition, Hanyang University , Seoul 04763, Republic of Korea.
Western Research Center, United States Department of Agriculture , Albany, California 94710, United States.
J Agric Food Chem. 2017 Nov 22;65(46):10011-10019. doi: 10.1021/acs.jafc.7b03764. Epub 2017 Nov 8.
Physiological properties of water-soluble exopolysaccharides (EPS) and residues after EPS removal (Res) from the probiotic kefir were determined in high-fat (HF) diet-fed C57BL/6J mice. EPS solutions showed rheological properties and lower viscosity compared to those of β-glucan (BG). EPS significantly suppressed the adipogenesis of 3T3-L1 preadipocytes in a dose-dependent manner. Mice were fed HF diets containing 5% EPS, 5% BG, 8% Res, or 5% microcrystalline cellulose (control) for 4 weeks. Compared with the control, EPS supplementation significantly reduced HF diet-induced body weight gain, adipose tissue weight, and plasma very-low-density lipoprotein cholesterol concentration (P < 0.05). Res and BG significantly reduced body weight gain; however, reduction in adipose tissue weight was not statistically significant, suggesting that the antiobesity effect of EPS occurs due to viscosity and an additional factor. EPS supplementation significantly enhanced abundance of Akkermansia spp. in feces. These data indicate that EPS shows significant antiobesity effects possibly via intestinal microbiota alterations.
在高脂（HF）饮食喂养的C57BL/6J小鼠中，测定了来自益生菌开菲尔的水溶性胞外多糖（EPS）及其去除EPS后的残余物（Res）的生理特性。与β-葡聚糖（BG）相比，EPS溶液表现出流变学特性且粘度较低。EPS以剂量依赖性方式显著抑制3T3-L1前脂肪细胞的脂肪生成。给小鼠喂食含5% EPS、5% BG、8% Res或5%微晶纤维素（对照）的高脂饮食4周。与对照组相比，补充EPS显著降低了高脂饮食诱导的体重增加、脂肪组织重量和血浆极低密度脂蛋白胆固醇浓度（P<0.05）。Res和BG显著降低了体重增加；然而，脂肪组织重量的降低无统计学意义，这表明EPS的抗肥胖作用是由于粘度和另一个因素所致。补充EPS显著增加了粪便中阿克曼氏菌属的丰度。这些数据表明，EPS可能通过改变肠道微生物群而显示出显著的抗肥胖作用。
