Dayangan Sayan Cemile, Tulmac Ozlem Banu, Karaca Gökhan, Ozkan Zehra Sema, Yalcin Selim, Devrim Tuba, Dindar Badem Nermin
a Faculty of Medicine , Kırıkkale University , Kırıkkale , Turkey.
Gynecol Endocrinol. 2018 Apr;34(4):309-313. doi: 10.1080/09513590.2017.1395836. Epub 2017 Oct 30.
The aim of this study is to investigate whether erythropoietin (EPO) can reduce the ovarian damage of cisplatin or not. Thirty, female, Wistar-Albino rats were used in the study. Control group (N = 10): Intraperitoneal saline infusion, Cisplatin group (N = 10): Intraperitoneal 7 mg/kg cisplatin, Cisplatin + EPO group (N = 10): Intraperitoneal 7 mg/kg cisplatin and subcutaneous 200 IU/kg/day EPO. Serum AMH concentrations were measured by enzyme-linked immunosorbent assay kit of AMH. Follicular counts were evaluated according to mean diameter of the follicles. Ovarian damage; including follicular cell degeneration, vascular congestion, hemorrhage, and inflammation was scored histologically using a graduated scale. Posttreatment AMH levels of cisplatin group were significantly lower than control and cisplatin + EPO groups. In cisplatin group, there was a significant decrement in posttreatment AMH level compared to pretreatment AMH level. The total damage score of cisplatin group was significantly higher than scores of control and cisplatin + EPO groups. The mean primordial follicle counts of control and cisplatin + EPO groups were significantly higher than that of cisplatin group (p = .007 and p = .003). The results of this study revealed that EPO administration to cisplatin chemotherapy could ameliorate the ovarian damage. Erythropoietin administration to chemotherapeutic agents might suggest to protect ovarian failure and infertility.
本研究的目的是调查促红细胞生成素（EPO）是否能减轻顺铂对卵巢的损伤。本研究使用了30只雌性Wistar - Albino大鼠。对照组（N = 10）：腹腔注射生理盐水；顺铂组（N = 10）：腹腔注射7 mg/kg顺铂；顺铂+ EPO组（N = 10）：腹腔注射7 mg/kg顺铂并皮下注射200 IU/kg/天EPO。采用AMH酶联免疫吸附测定试剂盒检测血清AMH浓度。根据卵泡的平均直径评估卵泡计数。对卵巢损伤，包括卵泡细胞变性、血管充血、出血和炎症，采用分级量表进行组织学评分。顺铂组治疗后的AMH水平显著低于对照组和顺铂+ EPO组。与治疗前的AMH水平相比，顺铂组治疗后的AMH水平有显著下降。顺铂组的总损伤评分显著高于对照组和顺铂+ EPO组。对照组和顺铂+ EPO组的原始卵泡平均计数显著高于顺铂组（p = 0.007和p = 0.003）。本研究结果表明，顺铂化疗时给予EPO可减轻卵巢损伤。对化疗药物给予促红细胞生成素可能提示对卵巢功能衰竭和不孕症有保护作用。
