Talebi Athar, Hayati Roodbari Nasim, Reza Sameni Hamid, Zarbakhsh Sam
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Int J Reprod Biomed. 2020 Jul 22;18(7):551-560. doi: 10.18502/ijrm.v13i7.7372. eCollection 2020 Jul.
Apigenin is a plant-derived flavonoid with antioxidative and antiapoptotic effects. Bone marrow stromal cells (BMSCs) are a type of mesenchymal stem cells (MSCs) that may recover damaged ovaries. It seems that apigenin may promote the differentiation of MSCs.
The aim of this study was to investigate the effect of coadministration of apigenin and BMSCs on the function, structure, and apoptosis of the damaged ovaries after creating a chemotherapy model with cyclophosphamide in rat.
For chemotherapy induction and ovary destruction, cyclophosphamide was injected intraperitoneally to 40 female Wistar rats (weighing 180-200 gr, 10 wk old) for 14 days. Then, the rats were randomly divided into four groups (n = 10/each): control, apigenin, BMSCs and coadministration of apigenin and BMSCs. Injection of apigenin was performed intraperitoneally and BMSC transplantation was performed locally in the ovaries. The level of anti-mullerian hormone serum by ELISA kit, the number of oocytes by superovulation, the number of ovarian follicles in different stages by H&E staining, and the expression of ovarian Bcl-2 and Bax proteins by western blot were assessed after four wk.
The results of serum anti-mullerian hormone level, number of oocytes and follicles, and Bcl-2/Bax expression ratio showed that coadministration of apigenin and BMSCs significantly recovered the ovarian function, structure, and apoptosis compared to the control, BMSC, and apigenin groups (p 0.001).
The results suggest that the effect of coadministration of apigenin and BMSCs is maybe more effective than the effect of their administrations individually on the recovery of damaged ovaries following the chemotherapy with cyclophosphamide in rats.
芹菜素是一种植物源黄酮类化合物，具有抗氧化和抗凋亡作用。骨髓基质细胞（BMSCs）是一种间充质干细胞（MSCs），可能会修复受损卵巢。芹菜素似乎可以促进间充质干细胞的分化。
本研究旨在探讨在大鼠中用环磷酰胺建立化疗模型后，联合应用芹菜素和骨髓基质细胞对受损卵巢的功能、结构和凋亡的影响。
为诱导化疗和破坏卵巢，对40只雌性Wistar大鼠（体重180 - 200克，10周龄）腹腔注射环磷酰胺，持续14天。然后，将大鼠随机分为四组（每组n = 10）：对照组、芹菜素组、骨髓基质细胞组和芹菜素与骨髓基质细胞联合应用组。腹腔注射芹菜素，并在卵巢局部进行骨髓基质细胞移植。四周后，通过酶联免疫吸附测定试剂盒评估抗苗勒管激素血清水平，通过超排卵评估卵母细胞数量，通过苏木精 - 伊红染色评估不同阶段的卵巢卵泡数量，并通过蛋白质免疫印迹法评估卵巢Bcl - 2和Bax蛋白的表达。
血清抗苗勒管激素水平、卵母细胞和卵泡数量以及Bcl - 2/Bax表达比值的结果表明，与对照组、骨髓基质细胞组和芹菜素组相比，联合应用芹菜素和骨髓基质细胞能显著恢复卵巢功能、结构和凋亡情况（p < 0.001）。
结果表明，在大鼠接受环磷酰胺化疗后，联合应用芹菜素和骨髓基质细胞对受损卵巢恢复的效果可能比单独应用它们的效果更显著。
