Research unit of Macromolecular Biochemistry and Genetic, Faculty of Sciences of Gafsa, 2112, Gafsa, Tunisia.
Common Services Unit for Research, Faculty of Sciences of Gafsa, 2112, Gafsa, Tunisia.
Lipids Health Dis. 2017 Oct 30;16(1):206. doi: 10.1186/s12944-017-0596-1.
Plant essential oils and phenolic compounds are widely used for their medicinal properties. Thus, the aim of this study is to evaluate the nutritional values, the chemical composition, antioxidant activity and anti-hemolytic effects of Pittosporum tobira seeds.
The aroma compounds were isolated using two methods (Headspace-solid phase microextraction (HS-SPME) and hydrodistillation (HD)) and analyzed by gas chromatography coupled with mass spectrometry (GC-MS). Bioactive phenolic compounds were identified by mean of high-performance liquid chromatography (HPLC-DAD). Reducing power, hydrogen peroxide (HO) scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays were used to investigate antioxidant activity. Anti-hemolytic activity was evaluated using HO-induced hemolysis of red blood cells (RBC).
Oxygenated sesquiterpenes, sesquiterpene hydrocarbons and oxygenated monoterpenes were the most volatile fractions identified by HD and HS-SPME coupled to GC-MS but their quality and amount were quite different according to the extraction methodology. The main phenolic compounds identified by HPLC were caffeic acid, followed by cinnamic acid and gallic acid. P. tobira seeds essential oils showed significant antioxidant activity in DPPH (IC value = 1.5 mg/mL), HO scavenging assay (IC value = 159.43 μg/mL) and reducing power test (IC value = 0.982 mg/mL) compared to methanolic extract. Moreover, the results revealed that the essential oil was able to protect RBC from hemolysis induced by HO. However, the methanolic extract had no effect on HO-induced hemolysis of RBC as compared to the essential oil and the standard vitamin C.
P. tobira may be used as a new natural source of antioxidant with therapeutic application in diseases caused by reactive oxygen species. Phytochemical Characterization and Biological Evaluation of Pittosporum tobira seeds.
植物精油和酚类化合物因其药用特性而被广泛应用。因此，本研究旨在评估叶柃种子的营养价值、化学成分、抗氧化活性和抗溶血作用。
采用顶空固相微萃取（HS-SPME）和水蒸馏（HD）两种方法分离香气化合物，并通过气相色谱-质谱联用（GC-MS）进行分析。采用高效液相色谱-二极管阵列检测（HPLC-DAD）法鉴定生物活性酚类化合物。采用还原力、过氧化氢（HO）清除和 2,2-二苯基-1-苦基肼（DPPH）自由基清除试验研究抗氧化活性。采用 HO 诱导红细胞（RBC）溶血评价抗溶血活性。
HD 和 HS-SPME 结合 GC-MS 鉴定出的含氧倍半萜、萜类烃和含氧单萜是最挥发的馏分，但它们的质量和数量根据提取方法有很大的不同。HPLC 鉴定的主要酚类化合物是咖啡酸，其次是肉桂酸和没食子酸。与甲醇提取物相比，叶柃种子精油在 DPPH（IC 值=1.5 mg/mL）、HO 清除试验（IC 值=159.43 μg/mL）和还原力试验（IC 值=0.982 mg/mL）中表现出显著的抗氧化活性。此外，结果表明，精油能够保护 RBC 免受 HO 诱导的溶血。然而，与精油和标准维生素 C 相比，甲醇提取物对 HO 诱导的 RBC 溶血没有影响。
叶柃可作为一种新的具有治疗活性氧相关疾病应用的抗氧化天然来源。叶柃种子的植物化学特征及生物学评价。
