Benhelima Abdelkader, Kaid-Omar Zohra, Hemida Houari, Benmahdi Tarek, Addou Ahmed
Laboratory of Science and Technology of Environment and development, urinary lithiasis group, faculty of science and technology, Abdelhamid Ibn Badis University, Mostaganem, Algeria.
Department of biolology, Faculty of Science, Dr. Tahar Moulay University, 20000, Saida, Algeria.
Afr J Tradit Complement Altern Med. 2016 Sep 29;13(6):204-214. doi: 10.21010/ajtcam.v13i6.30. eCollection 2016.
The purpose of the current investigation was to study the influences, preventive and diuretic, of L. seeds oil () on calcium oxalate (CaOx) urolithiasis induced in Wistar male rats.
Seeds of L. () were analysed for the evaluation of the concentration of oxalate and calcium. L. seeds oil is obtained by hydrodistillation and HPTLC densitometric method was adopted to determine the amount of thymoquinone (TQ) present. Thirty male Wistar rats were divided into 5 groups (=6). Group I, negative control, drank tap water. The other groups were II Positive control, III, IV and V received a treatment model inducing calcium oxalate urolithiasis for 28 days, using an aqueous solution involve 0.75% (EG) ethylene glycol and 1.0 % (AC) chloride ammonium. Rats in group III received in addition, 750 mg/kg Cystone from the beginning to the end of calculi induction experimentation. However, rats in Groups IV and V received () at 5 ml/kg b.w by gavage on days 1 to 28 and 15 to 28 days, respectively. On days 0, 7, 14, 21 and 28, body weights were measured and the 24-hour urine samples were accumulated and analysed for biochemical elements. On the 28 day, blood samples were collected for the estimation of serum parameters including creatinine, BUN and uric acid. All animals were sacrificed at the end of the experiment and the kidneys were detached for histopathological examination.
Administration of () at 5 ml/kg body weight/dose/day for 28 days exerts a protective effect by reducing significantly (p <0.01) urinary and serum rates of calcium, phosphate and oxalate. This preventive diet could increase the volume of urine excreted.
The nephroprotectrice and diuretic activity demonstrated by L. gives a scientific basis that approves their traditional use like a remedy against urolithiasis. L. Seeds oil CaOx: Calcium Oxalate; N.S: L.; HPTLC: High performance thin layer chromatography; TQ: Thymoquinone; N: Number; EG: Ethylene Glycol; AC: Chloride Ammonium BUN: Blood Urea Nitrogen; LD: Lethal Dose 50; b.w: body weight; H & E: Haematoxyline and Eosin; HPLC-UV:;Caph: calcium phosphate; FR: glomerular filtration rate.
本研究旨在探讨黑种草籽精油对雄性Wistar大鼠草酸钙结石的预防及利尿作用。
分析黑种草籽中草酸盐和钙的含量。采用水蒸馏法提取黑种草籽精油，采用高效薄层色谱密度法测定其中百里醌（TQ）的含量。将30只雄性Wistar大鼠分为5组（每组6只）。第一组为阴性对照组，饮用自来水。其他组中，第二组为阳性对照组，第三组、第四组和第五组采用含0.75%乙二醇（EG）和1.0%氯化铵（AC）的水溶液诱导草酸钙结石形成，诱导期为28天。第三组大鼠在结石诱导实验开始至结束期间，额外给予750mg/kg的Cystone。第四组和第五组大鼠分别在第1至28天和第15至28天，以5ml/kg体重的剂量经口灌胃给予黑种草籽精油。在第0、7、14、21和28天，测量大鼠体重，收集24小时尿液样本并分析其中的生化成分。在第28天，采集血液样本，检测血清肌酐、尿素氮和尿酸等参数。实验结束时处死所有动物，摘取肾脏进行组织病理学检查。
以5ml/kg体重/剂量/天的剂量给予黑种草籽精油28天，可显著降低（p<0.01）尿液和血清中钙、磷和草酸盐的含量，发挥保护作用。这种预防性饮食可增加尿量。
黑种草籽精油所表现出的肾保护和利尿活性为其传统上用于治疗尿路结石提供了科学依据。黑种草籽精油；CaOx：草酸钙；N.S：黑种草；HPTLC：高效薄层色谱；TQ：百里醌；N：数量；EG：乙二醇；AC：氯化铵；BUN：血尿素氮；LD：半数致死剂量；b.w：体重；H&E：苏木精和伊红；HPLC-UV：；Caph：磷酸钙；FR：肾小球滤过率。
