Zhang Yu, Lei Jiaojie, Zhang Yaxun, Liu Zhihai, Zhang Jianzhong, Yang Xinghua, Yang Jun, Yuan Libo
Key Lab of In-fiber Integrated Optics, Ministry Education of China, Harbin Engineering University, Harbin, China.
Guilin University of Electronic Technology, Guilin, China.
Sci Rep. 2017 Oct 30;7(1):14378. doi: 10.1038/s41598-017-14802-1.
The ability to arrange cells and/or microparticles into the desired pattern is critical in biological, chemical, and metamaterial studies and other applications. Researchers have developed a variety of patterning techniques, which either have a limited capacity to simultaneously trap massive particles or lack the spatial resolution necessary to manipulate individual particle. Several approaches have been proposed that combine both high spatial selectivity and high throughput simultaneously. However, those methods are complex and difficult to fabricate. In this article, we propose and demonstrate a simple method that combines the laser-induced convection flow and fiber-based optical trapping methods to perform both regular and special spatial shaping arrangement. Essentially, we combine a light field with a large optical intensity gradient distribution and a thermal field with a large temperature gradient distribution to perform the microparticles shaping arrangement. The tapered-fiber-based laser-induced convection flow provides not only the batch manipulation of massive particles, but also the finer manipulation of special one or several particles, which break out the limit of single-fiber-based massive/individual particles photothermal manipulation. The combination technique allows for microparticles quick accumulation, single-layer and multilayer arrangement; special spatial shaping arrangement/adjustment, and microparticles sorting.
将细胞和/或微粒排列成所需图案的能力在生物学、化学、超材料研究及其他应用中至关重要。研究人员已开发出多种图案化技术,但这些技术要么在同时捕获大量粒子方面能力有限,要么缺乏操纵单个粒子所需的空间分辨率。已经提出了几种同时兼具高空间选择性和高通量的方法。然而,这些方法复杂且难以制造。在本文中,我们提出并展示了一种简单的方法,该方法结合了激光诱导对流和基于光纤的光镊方法来进行规则和特殊的空间成形排列。从本质上讲,我们将具有大光强梯度分布的光场和具有大温度梯度分布的热场相结合来进行微粒的成形排列。基于锥形光纤的激光诱导对流不仅提供了对大量粒子的批量操纵,还实现了对特殊的单个或几个粒子的精细操纵,突破了基于单光纤的大量/单个粒子光热操纵的限制。该组合技术能够实现微粒的快速聚集、单层和多层排列、特殊空间成形排列/调整以及微粒分选。